Woon-Lam Susan Leung;Sarah Hymowitz;Robert F. Kelley;Mark O'Connell;Zahra Shahrokh;Laura Simmons;Roger Pai;Iphigenia Koumenis;Avi J. Ashkenazi
发明人:
Avi J. Ashkenazi,Sarah Hymowitz,Robert F. Kelley,Iphigenia Koumenis,Woon-Lam Susan Leung,Mark O'Connell,Roger Pai,Zahra Shahrokh,Laura Simmons,Mark OConnell
申请号：
US12928422
公开号：
US20120077740A1
申请日:
2010.12.10
申请国别(地区):
US
年份:
2012
代理人:
摘要：
Methods of making Apo-2 ligand and formulations of Apo-2 ligand using divalent metal ions are provided. Such divalent metal ions include zinc and cobalt which improve Apo-2 ligand trimer formation and stability. The crystal structure of Apo-2 ligand is also provided, along with Apo-2 ligand variant polypeptides identified using oligonucleotide-directed mutagenesis.
