Audrey LE GOUELLEC,Bertrand TOUSSAINT,Benoit POLACK
申请号：
US15397175
公开号：
US20170114319A1
申请日:
2017.01.03
申请国别(地区):
US
年份:
2017
代理人:
摘要：
A process for obtaining an adapted strain of Pseudomonas includes the following steps: deleting the genes ExoS, ExoT, aroA and lasl in an initial Pseudomonas strain cultivated in a LB medium； progressively cultivating this strain in a chemically defined medium based on a glucose minimal medium supplemented with magnesium and calcium； wherein the adapted strain presents the same toxicity and secretion capacities than the initial strain, and its doubling time when cultivated in the chemically defined medium is less than 60 minutes. An adapted strain is furthermore treated to become “killed but metabolically active.”
