BRIAN CAMPBELL,COLLEEN TAYLOR,DAMIAN CURTIS,DANIEL M. JOO,DAVID SESIN,FRISBY DAVIS SMITH,GERARDO BUENO SALAZAR,HONG ZHU,JONATHAN S. MARGOLIS,MAGALIE GUILHABERT GOYA,REED NATHAN ROYALTY,SHAOHUA GUAN,TA
申请号：
BR112015008045
公开号：
BR112015008045A2
申请日:
2013.10.11
申请国别(地区):
BR
年份:
2017
代理人:
摘要：
Patents streptomyces microflavus and methods of their use to control plant diseases and pests. The present invention relates to novel streptomyces microflavus strains and methods of their use for controlling diseases or pests in a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotine producing streptomyces strain in which the fermentation broth contains at least about 1 g / l gougerotine. The invention also relates to a method of producing a fermentation broth of a streptomyces strain of gougerotine production, wherein the fermentation broth contains at least about 1 g / l gougerotine, the method comprising cultivating the streptomyces strain. in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at an effective concentration to achieve a gougerotine concentration of at least 1 g / l. The present disclosure also relates to the molecular cloning of a streptomyces microflavus gougerotine biosynthetic gene cluster, and identification of individual genes in the gene cluster, as well as the proteins so encoded. a gougerotin gene cluster comprising 13 open reading frames (orfs) is located within a genetic localization of streptomyces microflavus.resumo patente de invenção: "cepas streptomyces microflavus e métodos de seu uso para controlar doenças e pestes de plantas". a presente invenção refere-se a novas cepas de streptomyces microflavus e métodos de seu uso para o controle de doenças ou pestes de uma planta. a invenção também se relaciona a um caldo de fermentação obtido pelo cultivo de uma cepa streptomyces de produção de gougerotina, no qual o caldo de fermentação contém pelo menos cerca de 1 g/l de gougerotina. a invenção também se relaciona a um método de produção de um caldo de fermentação de uma cepa streptomyces de produção de gougerotina, no qual o caldo de fermentação contém pelo menos cerca de 1 g/l de goug
