The invention provides the first tube holder to be used in the collection ofcerebrospinal fluid (CSF) by lumbar puncture for neurotransmitter testing. Theholder includes five holes designed to support the five 1.5ml Eppendorf tubesrequired for the procedure. The holder also includes a handle that can be usedby a right or left handed medical professional. The holder provides a methodtofill, to the required volume, each 1.5ml tube in sequence, without having toholdeach tube by hand. There is also an allowance at one end to enable the deviceto be suspended over a receptacle when not in active use.