METHOD FOR CARRYING OUT BIOENGENERY OF BONE FIBER USING MESENQUIMIAL TRUNK CELLS OF TEETH ASSOCIATED TO BIOMATERIALS AND BIOENGENERY KIT OF AUTOGENOUS BONE TISSUE
Is a method of bioengineering bone tissue using mesenchymal stem cells of tooth pulp associated with different biomaterials (e.G. Mesenchymal stem cells more combined with collagen /hydroxyapatite membranes and /or other polymers) and their use for regeneration And filling critical bone defects； in which it is used in the methodology of isolation, characterization and association of the mesenchymal stem cells with the different biomaterials enable bioengineering of bone tissue for any bone in the body； and thus,This methodology will allow direct application of this technology (linking stem cells from tooth pulp to biomaterials),Safely for the physician /dentist /patient to use in regenerative medicine. This tissue bioengineering strategy reduces morbidity and eliminates pain to obtain autogenous bone from other regions of the body for patients who need to perform bone grafts； therefore, it refers to a technology to describe a process for obtaining cells Mesenchymal torso of toothpaste under appropriate conditions for use in bioengineering of tissues,Which must be carried out in laboratories for good handling practice (cell technology centre type II) and how to associate these cells with different types of biomateries in good handling practice laboratories for delivery of the bone tissue bioengineering kit to the host Ital, medical or dental practitioners in a safe way for use in different bone reconstructions； and also, present the bone tissue bioengineering kit is composed of the appropriate combination of the biomaterial stem cells of tooth pulp. 0trata-se de um método de bioengenharia de tecido ósseo utilizando células tronco mesenquimais de polpa de dentes associadas a diferentes biomateriais (por exemplo, células tronco mesenquimais combinadas a membranas de colágeno/hidroxiapatita e/ou outros polímeros) e seu uso para regeneração e ou preenchimento de defeitos ósseos críticos； no qual, é utilizada na metodologia de isolamento, caracterização e associação das c
