Genetic Diversity of Legionella pneumophila Isolates from Artificial Water Sources in Brazil

• 全文链接
• 请求原文

作   者：

Ferrari, Dandrea Driely de Melo;  Lima, Solange Costa;  Teixeira, Raquel Lima Figueiredo;  Lopes, Marcia Quinhones Pires;  Vaconcellos, Sidra Ezidio Goncalves;  Machado Jr, Edson Silva;  Suffys, Philip Noel;  Gomes, Harrison Magdinier; 

作者机构：

Conforlab Engn Ambiental;  Inst Oswaldo Cruz Fiocruz; 

关键词：

STRAINS;  ABILITY;  EPIDEMIOLOGY;  SEQUENCE-BASED SCHEME; 

期刊名称：

Current Microbiology: An International Journal

i s s n：

0343-8651

年卷期：

2024 年 81 卷 6 期

页   码：

ARTN 165-

页   码：

摘   要：

Legionella pneumophila (Lp) is a Gram-negative bacterium found in natural and artificial aquatic environments and inhalation of contaminated aerosols can cause severe pneumonia known as Legionnaires' Disease (LD). In Brazil there is hardly any information about this pathogen, so we studied the genetic variation of forty Legionella spp. isolates obtained from hotels, malls, laboratories, retail centers, and companies after culturing in BCYE medium. These isolates were collected from various sources in nine Brazilian states. Molecular identification of the samples was carried out using Sequence-Based Typing (SBT), which consists of sequencing and analysis of seven genes (flaA, pilE, asd, mip, mompS, proA, and neuA) to define a Sequence Type (ST). Eleven STs were identified among 34/40 isolates, of which eight have been previously described (ST1, ST80, ST152, ST242, ST664, ST1185, ST1464, ST1642) and three were new STs (ST2960, ST2962, and ST2963), the former identified in five different cooling towers in the city of S & atilde;o Paulo. The ST1 that is widely distributed in many countries was also the most prevalent in this study. In addition, other STs that we observed have also been associated with legionellosis in other countries, reinforcing the potential of these isolates to cause LD in Brazil. Unfortunately, no human isolates could be characterized until presently, but our observations strongly suggest the need of surveillance implementation system and control measures of Legionella spp. in Brazil, including the use of more sensitive genotyping procedures besides ST.