University of Antwerp;
Crete;
Department of Biology;
1015 Lausanne;
INRAE;
Switzerland.;
78000 Versailles;
Universite Paris-Saclay;
Institut Jean-Pierre Bourgin (IJPB);
8008 Zurich;
University of Zurich;
Integrated Molecular Plant Physiology Research (IMPRES);
Hellenic Mediterranean University;
Heraklion;
Belgium;
University of Lausanne;
Switzerland;
2020 Antwerp;
Plant Biochemistry & Biotechnology Lab;
IINS;
Greece;
INRAE;
AgroParisTech;
Department of Plant Molecular Biology;
CIMRS UMR5297;
University of Bordeaux;
UR1268 BIA;
33000 Bordeaux;
Department of Plant Molecular Biology;
F-44300 Nantes;
Department of Plant and Microbial Biology & Zurich-Basel Plant Science Center;
Electron Microscopy Facility;
The Plant Signaling Mechanisms Laboratory;
France;
Switzerland. ;
Stavromenos PC 71410;
Department of Agriculture;
期刊名称：
Science
i s s n：
0036-8075
年卷期：
2023 年
382 卷
Nov.10 TN.6671 期
页 码：
719-725
页 码：
摘 要：
Assembly of cell wall polysaccharides into specific patterns is required for plant growth. A complex of RAPID ALKALINIZATION FACTOR 4 (RALF4) and its cell wall-anchored LEUCINE-RICH REPEAT EXTENSIN 8 (LRX8)-interacting protein is crucial for cell wall integrity during pollen tube growth, but its molecular connection with the cell wall is unknown. Here, we show that LRX8-RALF4 complexes adopt a heterotetrametric configuration in vivo, displaying a dendritic distribution. The LRX8-RALF4 complex specifically interacts with demethylesterified pectins in a charge-dependent manner through RALF4's polycationic surface. The LRX8-RALF4-pectin interaction exerts a condensing effect, patterning the cell wall's polymers into a reticulated network essential for wall integrity and expansion. Our work uncovers a dual structural and signaling role for RALF4 in pollen tube growth and in the assembly of complex extracellular polymers.
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