A novel selenite‐tolerant rhizosphere bacterium Wautersiella enshiensis sp. nov., isolated from Chinese selenium hyperaccumulator, Cardamine hupingshanensis

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作   者：

Linxi Yuan;  Zengrun Xia;  Chenyang He; 

作者机构：

Key Laboratory of Se‐enriched Products Development and Quality Control;  Department of Health and Environmental Sciences;  Xi′an Jiaotong‐Liverpool University;  Ministry of Agriculture and; 

关键词：

16S rRNA;  red nano‐Se;  Wautersiella enshiensis sp. nov;  taxonomy;  rhizosphere bacterium; 

期刊名称：

Journal of basic microbiology.

i s s n：

0233-111X

年卷期：

2023 年 63 卷 11 期

页   码：

1305-1315

页   码：

摘   要：

Abstract Selenium (Se) is a dietary essential trace element for humans with various physiological functions and it could also be accumulated by some plant species, like Astragalus bisulcatus, Stanleya pinnata, and Cardamine hupinshanensis. A novel Gram‐stain‐negative, facultatively anaerobic, selenite‐tolerant bacterium, designated strain YLX‐1T, was isolated from the rhizosphere of a Se hyperaccumulating plant, Cardamine hupingshanensis in Enshi, China. Phylogenetic analysis based on 16?S rRNA gene sequences indicated that strain YLX‐1T is a potential new species in the genus Wautersiella. Strain YLX‐1T could grow in the temperature range of 4–37°C (optimally at 28°C) and in the pH range of 5–9 (optimum pH 7), which also could tolerate Se up to 6000?mg Se/L via producing extracellular red nano‐Se with 100–300?nm size. However, it could predominantly accumulate selenocystine (SeCys2) in the cell under lower Se stress (1.5?mg Se/L). These results would help broaden our knowledge about the Se accumulation and transformation mechanism involved in rhizosphere bacteria like strain YLX‐1T in C. hupingshanensis. Based on polyphasic data, we propose the creation of the new species Wautersiella enshiensis sp. nov., strain YLX‐1T (?=?CCTCC M 2013671) which will be promising to produce nano‐Se as fertilizer, food additives or medicine.