Synthesis and characterization of copper oxide nanoparticles: its influence on corn (Z. mays) and wheat (Triticum aestivum) plants by inoculation of Bacillus subtilis
Daffodil International University;
Riphah International University;
University of Lahore;
Virtual University of Pakistan;
University of Agriculture;
King Saud University;
University of Okara;
Government College Women University;
University of Sargodha;
Prince Sattam Bin Abdulaziz University;
Banaras Hindu University;
Abstract Nanotechnology is now playing an emerging role in green synthesis in agriculture as nanoparticles (NPs) are used for various applications in plant growth and development. Copper is a plant micronutrient; the amount of copper oxide nanoparticles (CuONPs) in the soil determines whether it has positive or adverse effects. CuONPs can be used to grow corn and wheat plants by combining Bacillus subtilis. In this research, CuONPs were synthesized by precipitation method using different precursors such as sodium hydroxide (0.1?M) and copper nitrate (Cu(NO3)2) having 0.1?M concentration with a post-annealing method. The NPs were characterized through X-ray diffraction (XRD), scanning electron microscope (SEM), and ultraviolet (UV) visible spectroscopy. Bacillus subtilis is used as a potential growth promoter for microbial inoculation due to its prototrophic nature. The JAR experiment was conducted, and the growth parameter of corn (Z. mays) and wheat (Triticum aestivum) was recorded after 5?days. The lab assay evaluated the germination in JARs with and without microbial inoculation under CuONP stress at different concentrations (25 and 50?mg).