Gill histological and transcriptomic analysis provides insights into the response of spotted sea bass (Lateolabrax maculatus) to alkalinity stress

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作   者：

Zhang, Yonghang;  Wen, Haishen;  Liu, Yang;  Qi, Xin;  Sun, Donglei;  Zhang, Chong;  Zhang, Kaiqiang;  Zhang, Meizhao;  Li, Jifang;  Li, Yun; 

作者机构：

Minist Agr & Rural Affairs;  Ocean Univ China; 

关键词：

Alternative splicing;  LEUCISCUS-WALECKII;  ENVIRONMENTS;  INVOLVEMENT;  Alkalinity stress;  Histology;  FAMILY;  SIGNAL-TRANSDUCTION;  EVOLUTION;  Lateolabrax maculatus;  ORYZIAS-LATIPES;  RNA-Seq;  FISH;  GENOME-WIDE IDENTIFICATION;  EXPRESSION; 

期刊名称：

Aquaculture

i s s n：

0044-8486

年卷期：

2023 年 563 卷 Pt.1 期

页   码：

ARTN 738945-

页   码：

摘   要：

Spotted sea bass (Lateolabrax maculatus), possessing a superior adaptation ability to a wide range of salinities, is a valuable candidate to develop saline-alkaline tolerant fish strains in China, and therefore exploring the mech-anism of response to alkalinity environment is necessary to accelerate the molecular breeding program. In this study, gill morphological and transcriptomic profiles of spotted sea bass was characterized after alkalinity stress. Several morphological parameters of gill tissues were measured and quantified, showing the significantly his-tological changes after alkalinity challenge. RNA-Seq analysis identified a total of 2141 differentially expressed genes (DEGs) upon alkalinity exposure. Based on the soft clustering analysis, KEGG enrichment analysis and Gene Set Enrichment Analysis (GSEA), the up-regulated DEGs were significantly associated with cell-cell junc-tions and cell-ECM interactions (such as "ECM-receptor interaction", "Focal adhesion" and "Cell adhesion molecules"), signal transduction (including "Apelin signaling pathway", "MAPK signaling pathway", and "Hedgehog signaling pathway"), as well as ion transportation. Protein-Protein Interaction (PPI) analysis revealed that 13 genes including col6a1, itga9, itgb1, itgb4, pik3r2, ctnna2, kdrl, col6a1, fgf2, met, ptk2, itga8, and cdh2 were identified as hub genes. The down-regulated DEGs were significantly enriched in genetic information processing related categories, including "Cell cycle", "RNA polymerase", "RNA transport", "Spliceosome", "Ribosome", "Protein process in endoplasmic reticulum" and "Protein export". Among them, 11 genes including Pun, gapdh, fos, atf3, cebpb, egr1, aurka, aurkb, cdc20, mad2l1, and ccnb1 were recognized as hub genes. Additionally, totally 549 alkalinity-induced differential alternative splicing (DAS) genes were characterized, most of which belonged to skipped exon type and enriched in metabolic pathways and cell-cell junctions associated functional sets. Our study provides insights into the genetic basis for alkalinity adaptation, and will facilitate to select the regulatory elements potential applied for molecular breeding programs for the utilization of alkalinity waters.