CTnI diagnosis in myocardial infarction using G-quadruplex selective Ir(III) complex as effective electrochemiluminescence probe

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作   者：

Wu, Shujie;  Zou, Seyin;  Wang, Songen;  Li, Zongbing;  Ma, Dik-Lung;  Miao, Xiangmin; 

作者机构：

Guangdong Second Prov Gen Hosp;  Hong Kong Baptist Univ;  Jiangsu Normal Univ; 

关键词：

GOLD NANOPARTICLES;  SIGNAL;  Gold nanoparticles;  COLORIMETRIC ASSAY;  G-quadruplex;  IRIDIUM(III) COMPLEX;  Electrochemiluminescence;  LUMINESCENT DETECTION;  AMPLIFICATION;  cTnI detection;  Iridium(III) complex;  HYBRIDIZATION CHAIN-REACTION;  METAL-COMPLEXES;  MICRORNA; 

期刊名称：

Talanta: The International Journal of Pure and Applied Analytical Chemistry

i s s n：

0039-9140

年卷期：

2022 年 248 卷

页   码：

ARTN 123622-

页   码：

摘   要：

In this work, strong electrochemiluminescence (ECL) emission was achieved by using one type of the G-quadruplex selective iridium (III) complex as an efficient ECL signal probe. Based on the typical sandwich immunoreaction between the cardiac troponin-I antigen (cTnI) and its corresponding antibody, iridium (III) complex was introduced according to its specific interaction with G-quadruplex DNA that modified on the surface of negatively charged gold nanoparticles ((- )AuNPs), inducing an increased ECL signal, which was proportional to cTnI concentration. Based on of this, quantitative detection of cTnI could be realized in the range of 5.0 fg/mL100 ng/mL, with a detection limit of 1.67 fg/mL. Moreover, the proposed immunosensor was successfully applied for the diagnosis of cTnI in human serums from healthy individuals and acute myocardial infarction (AMI) patients, suggesting a great potential application value in the early diagnosis of AMI.