In-vivo Nitrate Reductase Activity Of Marine Angiosperm Halodule pinifolia (Miki) Hartog

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作   者：

Gunabalan Gunasekaran;  Saravanakumar Ayyappan;  Asaithambi Kalaiselvi;  Jeyapragash Danaraj; 

作者机构：

Annamalai University;  Department of Biotechnology;  Annamalai - 608 002;  Karpagam Academy of Higher Education;  Centre of Advanced Study in Marine Biology;  India;  Karpagam Academy of Higher Education;  Coimbatore- 641 021;  Tamil Nadu; 

关键词：

Pre-treatment;  Halodule pinifolia;  Enzyme;  Nitrate reductase;  Propanol; 

期刊名称：

Indian journal of environmental protection

i s s n：

0253-7141

年卷期：

2021 年 41 卷 3 期

页   码：

298-302

页   码：

摘   要：

Halodule pinifolia, a marine angiosperm collected from the Gulf of Mannar were assayed for nitrate reductase activity in order to determine the nitrate as a sole nitrogen source. Roots, rhizomes and leaves of H. pinifolia were used in the present study and the activity observed was very low. Pre-treatment of plant parts with varying concentration of nitrate did not induce higher nitrate reductase activity. Roots at a concentration of 100 μM NaNO_3 showed the maximum nitrate reductase activity (21.9 x 10~(-9) equiv. NO_2/h.gfr.wt), followed by the leaf part at a concentration of 100μM NaNO_3 (19.65 × 10~(-9) equiv. NO_2/h.gfr.wt). Nitrate reductase activity was not significantly influenced by varying the nitrate and propanol concentrations or pH of the assay medium. Present findings conclude that H. pinifolia does not use nitrate as a sole nitrogen source for their growth and might undergo other mechanisms, such as N_2 fixation and/or ammonium uptake to reach their growth and productivity in the marine environment.