Optimization of in vitro embryo production and zygote vitrification for the indigenous Vietnamese Ban pig: The effects of different in vitro oocyte maturation systems

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作   者：

Nguyen, Nhung Thi;  Bui, Nguyen Xuan;  Nguyen, Viet Linh;  Nguyen, Van Khanh;  Kikuchi, Kazuhiro;  Nguyen, Hiep Thi;  Nguyen, Hong Thi;  Nguyen, Hoang Thinh;  Dong, Quyen Van;  Chu, Hoang Ha;  Cuc, Ngo Thi Kim;  Somfai, Tamas; 

作者机构：

Fac Anim Sci;  Vietnam;  Natl Agr & Food Res Org;  Inst Agrobiol Sci;  Vietnam Natl Univ Agr;  Hanoi;  Inst Biotechnol;  Japan;  Natl Inst Anim Sci;  Ibaraki;  Natl Agr & Food Res Org NARO;  Tsukuba;  Inst Livestock & Grassland Sci;  Vietnam Acad Sci & Technol; 

关键词：

maturation;  embryos;  Ban pig oocytes;  medium; 

期刊名称：

Animal Science Journal

i s s n：

1344-3941

年卷期：

2020 年 91 卷 1 期

页   码：

摘   要：

The Vietnamese Ban pig is a precious genetic resource that needs to be preserved. In vitro embryo production from in vitro matured (IVM) oocytes is an important tool for the utilization of cryopreserved porcine sperm. The aim of this study was to compare two media for the IVM of Ban pig oocytes. Immature oocytes were subjected to IVM either in a non-defined (TCM-199 + pig follicular fluid) or in a defined base medium (POM + epidermal growth factor). At the end of IVM, the oocytes were in vitro fertilized (IVF) with frozen Ban sperm. Ten hours after IVF, the oocytes were either subjected to orcein staining to check fertilization and maturation status or cultured in vitro for 7 days. There was no difference between the two IVM media in terms of percentages of oocyte maturation and blastocyst production. However, the percentage of male pronuclear formation after IVF and the total cell numbers in blastocysts were higher with the defined system. Zygotes obtained by the two IVM systems survived vitrification at similar rates. In conclusion, the two IVM systems were both effective for the production of Ban pig embryos; however, better embryo quality was achieved with the defined one.