Synthetic secoisolariciresinol diglucoside (LGM2605) inhibits Libby amphibole fiber-induced acute inflammation in mice

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作   者：

Melpo Christofidou-Solomidou,;  Ralph A. Pietrofesa;  Kyewon Park;  Steven M. Albelda;  Kinta M. Serve;  Deborah E. Keil,;  Jean C. Pfau; 

作者机构：

Allergy;  Division of Pulmonary;  and Critical Care;  University of;  Department of Medicine; 

关键词：

Autoimmunity;  Asbestos;  Libby amphibole;  Inflammation;  Secoisolariciresinol diglucoside;  LGM2605; 

期刊名称：

Toxicology and Applied Pharmacology

i s s n：

0041-008X

年卷期：

2019 年 375 卷

页   码：

81-93

页   码：

摘   要：

Background: Exposure to the Libby amphibole (LA) asbestos-like fibers found in Libby, Montana, is associated with inflammatory responses in mice and humans, and an increased risk of developing mesothelioma, asbestosis, pleural disease, and systemic autoimmune disease. Flaxseed-derived secoisolariciresinol diglucoside (SDG) has anti-inflammatory, anti-fibrotic, and antioxidant properties. We have previously identified potent protective properties of SDG against crocidolite asbestos exposure modeled in mice. The current studies aimed to extend those findings by evaluating the imrnunomodulatory effects of synthetic SDG (LGM2605) on LA-exposed mice. Methods: Male and female C57BL/6 mice were given LGM2605 via gavage initiated 3 days prior to and continued for 3 days after a single intraperitoneal dose of LA fibers (200 mug) and evaluated on day 3 for inflammatory cell influx in the peritoneal cavity using flow cytometry. Results: LA exposure induced a significant increase (p < 0.0001) in spleen weight and peritoneal influx of white blood cells, all of which were reduced with LGM2605 with similar trends among males and females. Levels of peritoneal PMN cells were significantly (p < 0.0001) elevated post LA exposure, and were significantly (p < 0.0001) blunted by LGM2605. Importantly, LGM2605 significantly ameliorated the LA-induced mobilization of peritoneal B1a В cells. Conclusions: LGM2605 reduced LA-induced acute inflammation and WBC trafficking supporting its possible use in mitigating downstream LA fiber-associated diseases. Summary: Following acute exposure to Libby amphibole (LA) asbestos-like fibers, synthetic SDG (LGM2605), a small synthetic molecule, significantly reduced the LA-induced increase in spleen weight and peritoneal inflammation in C57BL/6 male and female mice. Our findings highlight that LGM2605 has immunomodulatory properties and may, thus, likely be a chemopreventive agent for LA-induced diseases.