Calendula officinalis extract/PCL/Zein/Gum arabic nanofibrous bio-composite scaffolds via suspension, two-nozzle and multilayer electrospinning for skin tissue engineering

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作   者：

Rad, Zahra Pedram;  Mokhtari, Javad;  Abbasi, Marjan; 

作者机构：

Univ Guilan;  Iran;  Dept Text Engn;  Fac Engn;  Rasht; 

关键词：

Calendula officinalis;  Gum Arabic;  Electrospinning;  Zein;  Skin tissue engineering;  Polycaprolactone; 

期刊名称：

International Journal of Biological Macromolecules: Structure, Function and Interactions

i s s n：

0141-8130

年卷期：

2019 年 135 卷

页   码：

530-543

页   码：

摘   要：

This work investigates the incorporation of the Calendula officinalis (C. officinalis) extract in electrospun fiber scaffolds composed of poly (e-caprolactone) (PCL), Zein and gum arabic (GA). Three methods of electrospinning were used: suspension electrospinning, in which C. officinalis extract was directly added in the PCL/Zein/GA solution, two-nozzle electrospinning, in which hybrid PCL/Zein/GA and PCL/C. officinalis nanofibrous layers were prepared by two syringes and multilayer electrospinning, in which layer-by-layer scaffold was fabricated of PCL/Zein/GA and PCL/C.officinalis nanofibrous mats. SEM micrographs of fabricated scaffolds depicted beadless nanofibers with interconnected pores. The PCL/Zein/GA/C.officinalis scaffolds possess good hydrophilicity with high porosity (about 80%) and also exhibited desirable mechanical properties and suitable degradability for skin tissue engineering. Multilayer produced scaffold showed more tensile strength than other C. officinalisloaded PCL/Zein/GA scaffolds. In vitro C.officinalis release exposed gradual and sustained release behavior for fabricated scaffold by multilayer electrospinning. The results of MTT analysis and SEM images confirmed that PCL/Zein/GA/C.officinalis nanocomposite scaffold had favorable proliferation and adhesion against fibroblast cell as compared to PCL/Zein/GA scaffold for regenerating skin. The C officinalis-loaded PCL/Zein/GA scaffold indicated better antibacterial properties and biocompatibility than PCL/Zein/GA scaffold. The results confirmed that C officinalis-loaded PCL/Zein/GA nanocomposite scaffolds would be desirable biomaterial for skin regeneration. (C) 2019 Elsevier B.V. All rights reserved.