Proteomic and functional analysis of zebrafish after administration of antimicrobial peptide epinecidin-1.

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作   者：

Huang TsuiChin;  Chen JyhYih; 

作者机构：

Institute of Cellular and Organismic Biology;  Academia Sinica;  23-10 Dahuen Rd.;  Ilan 262;  Ph.D. Program for Cancer Biology and Drug Discovery;  Marine Research Station;  Taipei Medical University and Academia Sinica;  Jiaushi;  Taiwan;  College of Medical Science and Technology; 

关键词：

Proteomic;  Epinecidin-1;  Zebrafish;  Antimicrobial peptide; 

期刊名称：

Fish & Shellfish Immunology

i s s n：

1050-4648

年卷期：

2013 年 34 卷 2 期

页   码：

593-598

页   码：

摘   要：

Antimicrobial peptides (AMPs) play important roles in innate immunity. One such AMP, epinecidin-1, exhibits antibacterial effects in zebrafish. In the current study, we aimed to identify the antimicrobial-associated proteins affected by epinecidin-1 treatment, and to unravel the underlying antimicrobial molecular mechanisms of epinecidin-1. We analyzed proteome changes in epinecidin-1-treated zebrafish using two-dimensional electrophoresis (2DE) coupled to mass spectrometry. Several differentially expressed proteins were identified, some of which were validated by real-time quantitative RT-PCR. The differentially expressed proteins were mapped onto Ingenuity Pathway Analysis canonical pathways, to construct a possible protein-protein interacting network regulated by epinecidin-1; this network suggested a potential role of epinecindin-1 in cytoskeletal assembly and organization. Our findings imply that epinecidin-1 may stabilize the cytoskeleton network in host cells, thereby promoting resistance to bacterial infection.