Differential polypeptide display: the search for the elusive target

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作   者：

Stefan Wittke;  Thorsten Kaiser;  Harald Mischak; 

作者机构：

Mosaiques Diagnostics and Therapeutics AG;  Germany;  Hannover; 

关键词：

reviews;  Data processing;  polypeptides; 

期刊名称：

Journal of chromatography, B. Analytical technologies in the biomedical and life sciences

i s s n：

1570-0232

年卷期：

2004 年 803 卷 1 期

页   码：

17-26

页   码：

摘   要：

Proteomics, as a tool to identify proteins in biological samples, is gaining rapidly importance in the postgenomic era. Here we discuss the current and potential role of different techniques in the field of proteomics such as two-dimensional gel electrophoresis off-line coupled to MALDI-MS (2D-PAGE-MALDI-MS), high performance liquid chromatography mass spectrometry (HPLC-MS), surface enhanced laser desorption/ionization mass spectrometry (SELDI-MS) and a newly developed technique, capillary electrophoresis mass spectrometry (CE-MS). The developments of the last years are presented discussed.