Coactivator MBF1 preserves the redox-dependent AP-1 activity during oxidative stress in Drosophila.

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作   者：

Jindra M;  Gaziova I;  Uhlirova M;  Okabe M;  Hiromi Y;  Hirose S; 

作者机构：

Ceske Budejovice;  University of South Bohemia;  Czech Republic.;  Department of Molecular Biology; 

关键词：

Drosophila Proteins;  反式作用因子;  Drosophila;  Trans-Activators;  果蝇属;  Transcription Factor AP-1;  转录因子AP-1; 

期刊名称：

EMBO Journal

i s s n：

0261-4189

年卷期：

2004 年 23 卷 17 期

页   码：

3538-3547

页   码：

摘   要：

Basic leucine zipper proteins Jun and Fos form the dimeric transcription factor AP-1, essential for cell differentiation and immune and antioxidant defenses. AP-1 activity is controlled, in part, by the redox state of critical cysteine residues within the basic regions of Jun and Fos. Mutation of these cysteines contributes to oncogenic potential of Jun and Fos. How cells maintain the redox-dependent AP-1 activity at favorable levels is not known. We show that the conserved coactivator MBF1 is a positive modulator of AP-1. Via a direct interaction with the basic region of Drosophila Jun (D-Jun), MBF1 prevents an oxidative modification (S-cystenyl cystenylation) of the critical cysteine and stimulates AP-1 binding to DNA. Cytoplasmic MBF1 translocates to the nucleus together with a transfected D-Jun protein, suggesting that MBF1 protects nascent D-Jun also in Drosophila cells. mbf1-null mutants live shorter than mbf1+ controls in the presence of hydrogen peroxide (H2O2). An AP-1-dependent epithelial closure becomes sensitive to H2O2 in flies lacking MBF1. We conclude that by preserving the redox-sensitive AP-1 activity, MBF1 provides an advantage during oxidative stress.