Molecular characterization of cultivars of Capsicum annuum L. and Capsicum frutescens L. (Solanaceae) from Valle del Cauca, Colombia by SSR-HRM technique

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作   者：

Viafara-Vega, Ronald A.;  Cifuentes, Hector G.;  Cardenas-Henao, Heiber; 

作者机构：

Univ Valle; 

关键词：

Capsicum genetic resource;  Microsatellite markers;  Genetic variation;  Artificial selection;  Genetic diversity; 

期刊名称：

Genetic Resources and Crop Evolution

i s s n：

0925-9864

年卷期：

2025 年 72 卷 4 期

页   码：

4539-4550

页   码：

摘   要：

The genus Capsicum includes plants known as chili, paprika or chili peppers, which are widely cultivated throughout the world. Its cultivation is of great importance because its fruits are widely used in gastronomy and industry. In Colombia, the species C. annuum, C. chinense and C. frutescens are cultivated. However, the Colombian production of chili peppers has not yet been recognized at the global level. Hence, in order to explore the genetic diversity and variation present in this genetic resource, it is important to molecularly characterize the material used in crops in Colombia. Thus, this study focused on the estimation of genetic diversity and variation of cultivated lines of C. annuum and C. frutescens species in Valle del Cauca using microsatellite markers and High-Resolution Melting (HRM) technology. No heterozygous individuals were found in the evaluated cultivars of either species. This is consistent with the mostly autogamous mode of reproduction of the genus and the selection process to which this genetic resource has been subjected. The allelic diversity of the cultivars was low, with most loci having a maximum of 3 alleles. However, for both species, the original cultivar maintained greater diversity than the derived cultivar. The analysis of molecular variance (AMOVA) identified the comparison of individuals within lines as the level with the highest variation (55% and 75%). This was followed by the variation between the original line (before selection) and the derived lines (37.25 and 11.6%). Furthermore, three useful microsatellite systems were identified for the differentiation of individuals of three species (C. annuum, C. frutescens and C. chinense) by HRM. Finally, the TL-8 line of C. frutescens is postulated for agronomic evaluation in different environments due to its genetic homogeneity, while the original lines of both species still have genetic variation to continue a breeding program.