Dominulin A and B: Two new antibacterial peptides identified on the cuticle and in the venom of the social paper wasp Polistes dominulus using MALDI-TOF, MALDI-TOF/TOF, and ESI-ion trap

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作   者：

Turillazzi S;  Mastrobuoni G;  Dani FR;  Moneti G;  Pieraccini G;  la Marca G;  Bartolucci G;  Perito B;  Lambardi D;  Cavallini V; 

作者机构：

Univ Florence;  Italy;  I-50100 Florence;  Dipartimento Sci Farmaceut;  Dipartimento Biol Anim & Genet;  I-50125 Florence;  Ctr Interdipartimentale Spettrometria Massa;  I-50121 Florence; 

关键词：

CELL DEGRANULATING PEPTIDE;  BIOLOGICAL CHARACTERIZATION;  HYMENOPTERA;  INSECT IMMUNITY;  LARVAL SALIVA;  GERMINATION;  MASTOPARAN;  HORNET; 

期刊名称：

Journal of the American Society for Mass Spectrometry

i s s n：

1044-0305

年卷期：

2006 年 17 卷 3 期

页   码：

376-383

页   码：

摘   要：

Two new antibacterial peptides, denominated as Dominulin A and B, have been found on the cuticle and in the venom of females of the social paper wasp Polistes dominulus. The amino acidic sequence of the two peptides, determined by mass spectrometry, is INWKKIAE VGGKIL SSL for Dominulin A (MW = 1854 Da) and INWKKIAEIGKQVL SAL (MW = 1909 Da) for Dominulin B. Their presence on the cuticle was confirmed using MALDI-TOF by means of micro-extractions and direct analyses on body parts. The presence in the venom and the primary structure of the dominulins suggest their classification in the mastoparans, a class of peptides found in the venom of other Aculeate hymenoptera. Their antimicrobial action against Gram+ and Gram- bacteria fits in the range of the best natural antimicrobial peptides. Dominulins can represent an important defense of the colony of Polistes dominulus against microbial pathogens.