Comprehensive evaluation of the capacities of microbial cell factories

• 全文链接

微生物细胞工厂能力的全面评估

关键词：

来源：

Nature Communications

全文链接：

//agri.nais.net.cn/topic/downloadFile/80654d82-f457-416a-b964-19434d22c9b3

来源地址：

https://www.nature.com/articles/s41467-025-58227-1#Abs1

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2025-03-24

摘要：

Systems metabolic engineering is facilitating the development of high-performing microbial cell factories for producing chemicals and materials. However, constructing an efficient microbial cell factory still requires exploring and selecting various host strains, as well as identifying the best-suited metabolic engineering strategies, which demand significant time, effort, and costs. Here, we comprehensively evaluate the capacities of various microbial cell factories and propose strategies for systems metabolic engineering steps, including host strain selection, metabolic pathway reconstruction, and metabolic flux optimization. We analyze the metabolic capacities of five representative industrial microorganisms as cell factories for the production of 235 different bio-based chemicals and suggest the most suitable host strain for the corresponding chemical production. To improve the innate metabolic capacity by constructing more efficient metabolic pathways, heterologous metabolic reactions, and cofactor exchanges are systematically analyzed. Additionally, we present metabolic engineering strategies, which include up- and down-regulation target reactions, for the improved production of chemicals. Altogether, this study will serve as a comprehensive resource for the systems metabolic engineering of microorganisms in the bio-based production of chemicals.