Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection

• 全文链接

番茄褐皱果病毒的生物学和分子表征及四重RT-PCR检测的发展

关键词：

来源：

Journal of Integrative Agriculture

全文链接：

//agri.nais.net.cn/topic/downloadFile/59c37458-14b8-4647-bb62-8aff59ea2de8

来源地址：

http://www.chinaagrisci.com/Jwk_zgnykxen/fileup/PDF/JIA-2020-0356.pdf

资源所属：

设施园艺专题

类型：

学术文献

语种：

英语

原文发布日期：

2020-06-11

摘要：

Tomato brown rugose fruit virus (ToBRFV) is a novel tobamovirus firstly reported in 2015 and poses a severe threat to the tomato industry.  So far, it has spread to ten countries in America, Asia, and Europe.  In 2019, we identified the occurrence of ToBRFV in Shandong Province (ToBRFV-SD), China.  Here, we showed ToBRFV-SD induce mild to severe mosaic and blistering on leaves, necrosis on sepals and pedicles, deformation, yellow spots, and brown rugose necrotic lesions on fruits.  ToBRFV-SD induced distinct symptoms on plants of tomato, Capsicum annumm, and Nicotiana benthamiana, and caused latent infection on plants of Solanum tuberosum, Solanum melongena, and N. tabacum cv. Zhongyan 102.  All the 50 tomato cultivars tested were highly sensitive to ToBRFV-SD.  The complete genomic sequence of ToBRFV-SD shared the highest nucleotide and amino acid identities with isolate IL from Israel.  In the phylogenetic tree constructed with the complete genomic sequence, all the ToBRFV isolates were clustered together and formed a sister branch with that of tobacco mosaic virus (TMV).  Furthermore, we developed a quadruplex RT-PCR system that could differentiate ToBRFV from TMV, tomato mosaic virus, and tomato spotted wilt virus, all of which are economically important viruses infecting tomato.  These results increase our understanding of the biological and molecular characteristics of ToBRFV and provide an efficient detection method for mixed infections, which will be helpful in the management of ToBRFV.