Synthetic Biology in Natural Product Biosynthesis

• 全文链接

天然产物生物合成中的合成生物学

关键词：

来源：

Chemical Reviews

全文链接：

//agri.nais.net.cn/topic/downloadFile/1f55c9f5-950c-4961-9aee-70db503b82ce

来源地址：

https://pubs.acs.org/doi/10.1021/acs.chemrev.4c00567

资源所属：

饲料用酶工程

类型：

学术文献

语种：

英语

原文发布日期：

2025-03-21

摘要：

Synthetic biology has played an important role in the renaissance of natural products research during the post-genomics era. The development and integration of new tools have transformed the workflow of natural product discovery and engineering, generating multidisciplinary interest in the field. In this review, we summarize recent developments in natural product biosynthesis from three different aspects. First, advances in bioinformatics, experimental, and analytical tools to identify natural products associated with predicted biosynthetic gene clusters (BGCs) will be covered. This will be followed by an extensive review on the heterologous expression of natural products in bacterial, fungal and plant organisms. The native host-independent paradigm to natural product identification, pathway characterization, and enzyme discovery is where synthetic biology has played the most prominent role. Lastly, strategies to engineer biosynthetic pathways for structural diversification and complexity generation will be discussed, including recent advances in assembly-line megasynthase engineering, precursor-directed structural modification, and combinatorial biosynthesis.