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Data from: Reverse genetics in the tidepool: knockdown of target gene expression via RNA interference in the copepod Tigriopus californicus
负责人:
关键词:
RNA interference;gene suppression;Copepoda;copepod;heat-shock protein;qPCR;gene expression;Tigriopus californicus;Ct values
DOI:
doi:10.5061/dryad.5cb17
摘要:
. The protocol was examined on five genes of interest, and for each, at least 50% knock-down in expression was achieved. While knock-down levels did not reach
Data from: Amino acid-specific δ15N trophic enrichment factor in fish fed with formulated diets varying in protein quantity and quality
负责人:
Herzka, Sharon
关键词:
DOI:
doi:10.5061/dryad.843372d
摘要:
protein digestibility using well-established protocols that do not change AA profiles. Growth rates were higher in diets with higher protein content, and isotopic
Data from: A novel protocol for studying bee cognition in the wild
负责人:
Muth, Felicity
关键词:
behaviour cognition field bumblebee honeybee Bombus Apis
DOI:
doi:10.5061/dryad.c2k5t
摘要:
in characteristics such as life history strategy, degree of sociality, and dietary specialization. Yet the limited number of protocols currently available for studying
Data from: Sampling strategies for delimiting species: genes, individuals, and populations in the Liolaemus elongatus-kriegi complex (Squamata
负责人:
关键词:
species boundaries;Mitochondrial DNA;nested-clade analysis;phylogeny;Liolaemus;lizards;sampling design
DOI:
doi:10.5061/dryad.836
摘要:
challenges for analysis when they span intra and inter-specific levels of divergence. Protocols have been designed to combine methods of analysis
Data from: From trial to implementation, bringing team-based learning online – Duke-NUS Medical School’s response to the COVID-19 pandemic
负责人:
关键词:
Covid-19;pandemic
DOI:
doi:10.5061/dryad.4mw6m907n
摘要:
module for home-based learning in response to the pandemic in 2020. These protocols were modified over the weeks, guided by feedback from students
Data from: Temporal variation in antibiotic environments slows down resistance evolution in pathogenic Pseudomonas aeruginosa
负责人:
关键词:
Experimental evolution;Evolutionary Medicine;Pseudomonas aeruginosa;Adaptation
DOI:
doi:10.5061/dryad.c867b
摘要:
of resistance. Here, we evaluated sequential treatment protocols using two fully independent laboratory-controlled evolution experiments with the human pathogen
Data from: Does kin selection moderate sexual conflict in Drosophila?
负责人:
关键词:
familiarity;sexual conflict;Drosophila;kin selection;mate harm;drosophila melanogaster;cooperation
DOI:
doi:10.5061/dryad.q6ds5
摘要:
of D. melanogaster using protocols very similar to those in the two previous studies. Our experiments show no evidence for a reduction in mate harm based upon either
Data from: Metabarcoding and mitochondrial metagenomics of endogean arthropods to unveil the mesofauna of the soil
负责人:
关键词:
Soil biodiversity;Mesofauna;Next Generation Sequencing
DOI:
doi:10.5061/dryad.5t5s1
摘要:
processing to obtain ‘clean’ DNA extractions of arthropod mesofauna from the soil. In addition, we developed and tested HTS protocols for the characterisation
Data from: Comparative evaluation of potential indicators and temporal sampling protocols for monitoring genetic erosion
负责人:
关键词:
conservation genetics;Simulation;Theoretical Population Genetics;Holocene;Conservation Biology;population dynamics
DOI:
doi:10.5061/dryad.2m397
摘要:
of knowledge regarding which genetic metrics, temporal sampling protocols, and genetic markers are sufficiently sensitive and robust, on conservation-relevant
Data from: Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae
负责人:
关键词:
Environmental DNA;Chironomidae;DNA Barcoding;insects;species identification;Community Ecology;Invertebrates;COI;Ecological Genetics;Aquatic Macroinvertebrates
DOI:
doi:10.5061/dryad.tf6r4
摘要:
e, we compare the relative effectiveness of five different DNA extraction protocols and direct PCR in isolation of DNA from chironomid pupal exuviae. Chiro

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