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Data from: High-throughput molecular identification of fish eggs using multiplex suspension bead arrays
负责人:
Gleason, Lani U
关键词:
fish egg identification DNA Barcoding suspension bead arrays Fish
DOI:
doi:10.5061/dryad.8p425
摘要:
. The amplicons were then hybridized to the bead array and after addition of a reporter fluorophore, samples were analyzed by flow cytometry with Luminex 100
Data from: Evolution and phylogeography analysis of diploid and polyploid Misgurnus anguillicaudatus populations across China
负责人:
Zhong, Jia
关键词:
polyploidy phylogeography population genetics geographic distribution M. anguillicaudatus
DOI:
doi:10.5061/dryad.n3s0p79
摘要:
geographical populations in China, their ploidy levels were determined using flow cytometry. Two mitochondrial markers (cytb and CR) were then used for phylogeographic
Data from: The small nuclear genomes of Selaginella are associated with a low rate of genome size evolution
负责人:
关键词:
Selaginella
DOI:
doi:10.5061/dryad.16n4q
摘要:
vascular plant clades in a comparative phylogenetic framework. For the analyses, we collected 29 new flow cytometry estimates of haploid genome size in Selaginella
Data from: Loss of murine Paneth cell function alters the immature intestinal microbiome and mimics changes seen in neonatal necrotizing enterocolitis
负责人:
McElroy, Steven J.
关键词:
NEC cecal microbiome
DOI:
doi:10.5061/dryad.1h5183k
摘要:
, electron microscopy, flow cytometry, and real time RT-PCR. Cecal samples were analyzed at various time points to determine the effects of Paneth cell depletion
Data from: Molecular analyses identify hybridization-mediated nuclear evolution in newly discovered fungal hybrids
负责人:
关键词:
Heterobasidion irregulare;Heterobasidion occidentale;hybridization
DOI:
doi:10.5061/dryad.6t7sh85
摘要:
s increased allelic diversity by generating novel polymorphisms unreported in either parental species. Sequence data and ploidy analysis through flow cytometry
Data from: The predictability of a lake phytoplankton community, over time-scales of hours to years
负责人:
关键词:
cell density;Cyanobacteria;phytoplankton;time series;Flow cytometry;microscopy
DOI:
doi:10.5061/dryad.r4454
摘要:
Forecasting changes to ecological communities is one of the central challenges in ecology. However, nonlinear dependencies, biotic interactions and data limitations have limited our ability to assess how predictable communities are. We used a machine learning approach and environmental monitoring data (biological, physical and chemical) to assess the predictability of phytoplankton cell density in one lake across an unprecedented range of time scales. Communities were highly predictable over hours to months: model R2 decreased from 0.89 at 4 hours to 0.75 at 1 month, and in a long-term dataset lacking fine spatial resolution, from 0.46 at 1 month to 0.32 at 10 years. When cyanobacterial and eukaryotic algal cell density were examined separately, model-inferred environmental growth dependencies matched laboratory studies, and suggested novel trade-offs governing their competition. High-frequency monitoring and machine learning can help elucidate the mechanisms underlying ecological dynamics and set prediction targets for process-based models.
Data from: No evidence that sex and transposable elements drive genome size variation in evening primroses
负责人:
关键词:
mating system;PTH;Oenothera;functional asexuality;repetitive DNA
DOI:
doi:10.5061/dryad.7mf4k
摘要:
. We determined genome size using flow cytometry for 30 species that vary in genetic system and find that variation in sexual/asexual reproduction cannot explain
Data from: CRISPR-Cas9 mediated CD133 knockout inhibits colon cancer invasion through reduced epithelial-mesenchymal transition
负责人:
关键词:
Vimentin;Human colon cancer cell line;cancer stem cell;Kangwon-do;CRISPR-Cas9;colon cancer;STAT3;CD133;Wonju
DOI:
doi:10.5061/dryad.8rm2dn0
摘要:
carrying CD133 gRNA and purified cell by culturing single cell colonies. CD133knockout cells was validated by western blot and flow cytometry analysis
Data from: Polyploidy and microsatellite variation in the relict tree Prunus lusitanica L.: how effective are refugia in preserving genotypic diversity of clonal taxa?
负责人:
关键词:
Prunus lusitanica;phylogeography;Angiosperms;population dynamics;Population Genetics - Empirical
DOI:
doi:10.5061/dryad.g9s2k
摘要:
of three subspecies: mainland lusitanica (Iberian Peninsula and Morocco), and island azorica (Azores) and hixa (Canary Islands and Madeira). Flow cytometry

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