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Data from: High-throughput molecular identification of fish eggs using multiplex suspension bead arrays
- 负责人:
- Gleason, Lani U
- DOI:
- doi:10.5061/dryad.8p425
- 摘要:
- . The amplicons were then hybridized to the bead array and after addition of a reporter fluorophore, samples were analyzed by flow cytometry with Luminex 100
Data from: Evolution and phylogeography analysis of diploid and polyploid Misgurnus anguillicaudatus populations across China
- 负责人:
- Zhong, Jia
- DOI:
- doi:10.5061/dryad.n3s0p79
- 摘要:
- geographical populations in China, their ploidy levels were determined using flow cytometry. Two mitochondrial markers (cytb and CR) were then used for phylogeographic
Data from: The small nuclear genomes of Selaginella are associated with a low rate of genome size evolution
- 负责人:
- 关键词:
- Selaginella
- DOI:
- doi:10.5061/dryad.16n4q
- 摘要:
- vascular plant clades in a comparative phylogenetic framework. For the analyses, we collected 29 new flow cytometry estimates of haploid genome size in Selaginella
Data from: Loss of murine Paneth cell function alters the immature intestinal microbiome and mimics changes seen in neonatal necrotizing enterocolitis
- 负责人:
- McElroy, Steven J.
- 关键词:
- NEC cecal microbiome
- DOI:
- doi:10.5061/dryad.1h5183k
- 摘要:
- , electron microscopy, flow cytometry, and real time RT-PCR. Cecal samples were analyzed at various time points to determine the effects of Paneth cell depletion
Data from: Molecular analyses identify hybridization-mediated nuclear evolution in newly discovered fungal hybrids
- 负责人:
- DOI:
- doi:10.5061/dryad.6t7sh85
- 摘要:
- s increased allelic diversity by generating novel polymorphisms unreported in either parental species. Sequence data and ploidy analysis through flow cytometry
Data from: The predictability of a lake phytoplankton community, over time-scales of hours to years
- 负责人:
- DOI:
- doi:10.5061/dryad.r4454
- 摘要:
- Forecasting changes to ecological communities is one of the central challenges in ecology. However, nonlinear dependencies, biotic interactions and data limitations have limited our ability to assess how predictable communities are. We used a machine learning approach and environmental monitoring data (biological, physical and chemical) to assess the predictability of phytoplankton cell density in one lake across an unprecedented range of time scales. Communities were highly predictable over hours to months: model R2 decreased from 0.89 at 4 hours to 0.75 at 1 month, and in a long-term dataset lacking fine spatial resolution, from 0.46 at 1 month to 0.32 at 10 years. When cyanobacterial and eukaryotic algal cell density were examined separately, model-inferred environmental growth dependencies matched laboratory studies, and suggested novel trade-offs governing their competition. High-frequency monitoring and machine learning can help elucidate the mechanisms underlying ecological dynamics and set prediction targets for process-based models.
Data from: No evidence that sex and transposable elements drive genome size variation in evening primroses
- 负责人:
- DOI:
- doi:10.5061/dryad.7mf4k
- 摘要:
- . We determined genome size using flow cytometry for 30 species that vary in genetic system and find that variation in sexual/asexual reproduction cannot explain
Data from: CRISPR-Cas9 mediated CD133 knockout inhibits colon cancer invasion through reduced epithelial-mesenchymal transition
- 负责人:
- DOI:
- doi:10.5061/dryad.8rm2dn0
- 摘要:
- carrying CD133 gRNA and purified cell by culturing single cell colonies. CD133knockout cells was validated by western blot and flow cytometry analysis
Data from: Polyploidy and microsatellite variation in the relict tree Prunus lusitanica L.: how effective are refugia in preserving genotypic diversity of clonal taxa?
- 负责人:
- 关键词:
- Prunus lusitanica;phylogeography;Angiosperms;population dynamics;Population Genetics - Empirical
- DOI:
- doi:10.5061/dryad.g9s2k
- 摘要:
- of three subspecies: mainland lusitanica (Iberian Peninsula and Morocco), and island azorica (Azores) and hixa (Canary Islands and Madeira). Flow cytometry
Data from: Ancestral remnants or peripheral segregates? Phylogenetic relationships of two narrowly endemic Euphrasia species (Orobanchaceae) from the eastern European Alps
- 负责人:
- DOI:
- doi:10.5061/dryad.2pm2j8j
- 摘要:
- own that E. inopinata and E. sinuata, whose diploid ploidy level is confirmed for all analysed individuals via flow cytometry, are phylogenetically closely