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Data from: Evaluating DNA degradation rates in faecal pellets of the endangered pygmy rabbit
- 负责人：
- 关键词：
- Brachylagus idahoensis;pygmy rabbit;genotyping error;noninvasive genetic sampling;DNA degradation
- DOI：
- doi:10.5061/dryad.722qb
- 摘要：
- for microsatellite loci declined rapidly from 80.0% to 42.7% between days 5 and 7, likely due to increased environmental temperature. Success rates for mtDNA amplification
Data from: Constraining the role of early land plants in Early Palaeozoic weathering and global cooling
- 负责人：
- DOI：
- doi:10.5061/dryad.6dh6g
- 摘要：
- controls. Phosphate weathering by mycorrhizal liverworts was amplified 9–13-fold over plant-free controls, compared with fivefold to sevenfold amplification
Data from: De novo discovery and multiplexed amplification of microsatellite markers for black alder (Alnus glutinosa) and related species using SSR
- 负责人：
- DOI：
- doi:10.5061/dryad.gg0jm
- 摘要：
- e tested for amplification, twelve of which were polymorphic in A. glutinosa. These twelve loci were successfully co-amplified in a single multi
Data from: Genetic identification of Iberian rodent species using both mitochondrial and nuclear loci: application to non-invasive sampling
- 负责人：
- Alves, Paulo C.
- DOI：
- doi:10.5061/dryad.fv21h
- 摘要：
- . Amplification success for the cyt-b and IRBP fragment was 85% and 43% in faecal and 88% and 64% in owl-pellet DNA extractions, respectively. The method allows
Data from: A study of the transit amplification divisions during spermatogenesis in Oncopeltus fasciatus to assess plasticity in sperm number
- 负责人：
- DOI：
- doi:10.5061/dryad.q57n9h7
- 摘要：
- of spermatogonial cells through the cell cycle during the transit amplification divisions. We demonstrated that diet affects the likelihood of a spermatocyst bei
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
- 负责人：
- DOI：
- doi:10.5061/dryad.n4cv3
- 摘要：
- amplification of barcoding regions, chimera formation is frequent and well documented. However, profiling of fungal communities typically uses the more variable
Data from: Confirmation of independent introductions of an exotic plant pathogen of Cornus species, Discula destructiva, on the east and west coast
- 负责人：
- Trigiano, Robert
- 关键词：
- Dogwood anthracnose microsatellite loci cross-amplification genetic diversity population bottleneck introduced pathogen
- DOI：
- doi:10.5061/dryad.k6j12
- 摘要：
- pathogen with independent introductions on the east and west coasts of North America. We also tested the cross-amplification of these microsatelli
Data from: Slippage of degenerate primers can cause variation in amplicon length
- 负责人：
- DOI：
- doi:10.5061/dryad.nk81st2
- 摘要：
- It is well understood that homopolymer regions should be avoided for primer binding to prevent off-target amplification. However, in metabarcoding
Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- r, we speculate that non-specific amplification is influenced by additional parameters, which lead to inaccuracies of in silico prediction. We performed
Data from: Partitioning between atmospheric deposition and canopy microbial nitrification into throughfall nitrate fluxes in a Mediterranean forest
- 负责人：
- DOI：
- doi:10.5061/dryad.3cq6127
- 摘要：
- s highlight the power of coupling microbial community analysis, functional gene amplification and stable isotope approaches to examine ecosystem-scale processes.
