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Data from: Minimizing polymerase biases in metabarcoding
负责人:
关键词:
Environmental DNA;Metabarcoding;trnL P6 loop;Bias;soil;eDNA
DOI:
doi:10.5061/dryad.k129c
摘要:
DNA metabarcoding is an increasingly popular method to characterize and quantify biodiversity in environmental samples. Metabarcoding approaches
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
负责人:
关键词:
DNA metabarcoding;Chimeric sequences;PCR bias;UCHIME;microbial ecology
DOI:
doi:10.5061/dryad.n4cv3
摘要:
The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template
Data from: Metabarcoding reveals diet diversity in an ungulate community in Thailand
负责人:
关键词:
Western Forest Complex;niche breadth;Metabarcoding;DNA Barcode;ForestGEO;Bovid;deer
DOI:
doi:10.5061/dryad.0cfxpnvx6
摘要:
e codified within a genetic barcode library and used in this study. This DNA library was supplemented with samples from forb and grass species
Data from: DNA barcoding of the ichthyofauna of Taal Lake, Philippines
负责人:
Aquilino, Sean Vincent L.
关键词:
DNA Barcoding Fish COI
DOI:
doi:10.5061/dryad.8416
摘要:
This study represents the first molecular survey of the ichthyofauna of Taal Lake and the first DNA barcoding attempt in Philippine fishes. Taal Lake
Data from: Metabarcoding using multiplexed markers increases species detection in complex zooplankton communities
负责人:
关键词:
Metabarcoding;Zooplankton;multigene;multiple primer pairs;COI;18S;Zooplankton
DOI:
doi:10.5061/dryad.m83jc20
摘要:
Metabarcoding combines DNA barcoding with high-throughput sequencing, often using one genetic marker to understand complex and taxonomically di
Data from: Influence of killing method on Lepidoptera DNA barcode recovery
负责人:
关键词:
Recent;freezing;killing method;Lepidoptera;ethyl acetate;cyanide
DOI:
doi:10.5061/dryad.18b1k
摘要:
The global DNA barcoding initiative has revolutionized the field of biodiversity research. Such large-scale sequencing projects require
Data from: Anacapa Toolkit: an environmental DNA toolkit for processing multilocus metabarcode datasets
负责人:
关键词:
Environmental DNA
DOI:
doi:10.5061/dryad.mf0126f
摘要:
1. Environmental DNA (eDNA) metabarcoding is a promising method to monitor species and community diversity that is rapid, affordable
Data from: From feces to data: a metabarcoding method for analyzing consumed and available prey in a bird-insect food web
负责人:
关键词:
dietary ecology;Anthropocene;Poecile montanus;metagenomics;frass;DNA Barcoding;Lepidoptera;insectivorous birds;Cyanistes caeruleus;Parus major;Fecal DNA;Poecile cinctus
DOI:
doi:10.5061/dryad.4f1n785
摘要:
size and the barcoding success, suggesting that the smallest frass samples do not contain enough larval DNA to be detected by high-throughput sequencing. To summar
Data from: Non-specific amplification compromises environmental DNA metabarcoding with COI
负责人:
关键词:
Environmental DNA;12S;Metabarcoding;Actinopterygii;COI;eDNA;primer design
DOI:
doi:10.5061/dryad.b8f6s44
摘要:
1. Metabarcoding extra-organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment
Data from: “Direct PCR” optimization yields a rapid, cost-effective, non-destructive, and efficient method for obtaining DNA barcodes without DNA extraction
负责人:
关键词:
ecosystem services;Chironomidae;DNA Barcoding;insects;Invertebrates
DOI:
doi:10.5061/dryad.4q4kf
摘要:
ely obtaining DNA barcodes from specimens requires many steps and thus time and money. Here, we promote a short-cut to DNA barcoding; i.e., a non-destructive

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