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Data from: Evaluating DNA degradation rates in faecal pellets of the endangered pygmy rabbit
负责人:
关键词:
Brachylagus idahoensis;pygmy rabbit;genotyping error;noninvasive genetic sampling;DNA degradation
DOI:
doi:10.5061/dryad.722qb
摘要:
for microsatellite loci declined rapidly from 80.0% to 42.7% between days 5 and 7, likely due to increased environmental temperature. Success rates for mtDNA amplification
Data from: Constraining the role of early land plants in Early Palaeozoic weathering and global cooling
负责人:
关键词:
Palaeozoic;biological weathering;land plant evolution;Early land plants;Palaeozoic climate;arbuscular mycorrhiza;Marchantia paleacea
DOI:
doi:10.5061/dryad.6dh6g
摘要:
controls. Phosphate weathering by mycorrhizal liverworts was amplified 9–13-fold over plant-free controls, compared with fivefold to sevenfold amplification
Data from: De novo discovery and multiplexed amplification of microsatellite markers for black alder (Alnus glutinosa) and related species using SSR
负责人:
关键词:
high throughput genotyping;Next-generation sequencing;short sequence repeats;Alnus incana;Alnus cordata;common alder;Alnus glutinosa;454 GS-FLX Titanium
DOI:
doi:10.5061/dryad.gg0jm
摘要:
e tested for amplification, twelve of which were polymorphic in A. glutinosa. These twelve loci were successfully co-amplified in a single multi
Data from: Genetic identification of Iberian rodent species using both mitochondrial and nuclear loci: application to non-invasive sampling
负责人:
Alves, Paulo C.
关键词:
Conservation Genetics Conservation Biology Ecological Genetics Mammals non-invasive sampling
DOI:
doi:10.5061/dryad.fv21h
摘要:
. Amplification success for the cyt-b and IRBP fragment was 85% and 43% in faecal and 88% and 64% in owl-pellet DNA extractions, respectively. The method allows
Data from: A study of the transit amplification divisions during spermatogenesis in Oncopeltus fasciatus to assess plasticity in sperm number
负责人:
关键词:
fertility;Oncopeltus fasciatus;spermatogenesis;sperm counts;Fecundity
DOI:
doi:10.5061/dryad.q57n9h7
摘要:
of spermatogonial cells through the cell cycle during the transit amplification divisions. We demonstrated that diet affects the likelihood of a spermatocyst bei
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
负责人:
关键词:
DNA metabarcoding;Chimeric sequences;PCR bias;UCHIME;microbial ecology
DOI:
doi:10.5061/dryad.n4cv3
摘要:
amplification of barcoding regions, chimera formation is frequent and well documented. However, profiling of fungal communities typically uses the more variable
Data from: Confirmation of independent introductions of an exotic plant pathogen of Cornus species, Discula destructiva, on the east and west coast
负责人:
Trigiano, Robert
关键词:
Dogwood anthracnose microsatellite loci cross-amplification genetic diversity population bottleneck introduced pathogen
DOI:
doi:10.5061/dryad.k6j12
摘要:
pathogen with independent introductions on the east and west coasts of North America. We also tested the cross-amplification of these microsatelli
Data from: Slippage of degenerate primers can cause variation in amplicon length
负责人:
关键词:
Metabarcoding;in silico
DOI:
doi:10.5061/dryad.nk81st2
摘要:
It is well understood that homopolymer regions should be avoided for primer binding to prevent off-target amplification. However, in metabarcoding
Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
负责人:
关键词:
DOI:
doi:10.5061/dryad.41ns1rnb7
摘要:
r, we speculate that non-specific amplification is influenced by additional parameters, which lead to inaccuracies of in silico prediction. We performed
Data from: Partitioning between atmospheric deposition and canopy microbial nitrification into throughfall nitrate fluxes in a Mediterranean forest
负责人:
关键词:
ammonia-oxidizing bacteria;stable isotopes;Metabarcoding;nitrogen fluxes;nitrogen deposition;Quercus ilex;ammonia-oxidizing archaea;Mediterranean forest
DOI:
doi:10.5061/dryad.3cq6127
摘要:
s highlight the power of coupling microbial community analysis, functional gene amplification and stable isotope approaches to examine ecosystem-scale processes.

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