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Data from: Phylogenetic affiliation of SSU rRNA genes generated by massively parallel sequencing: new insights into the freshwater protist diversity
- 负责人：
- 关键词：
- Protozoa;protist
- DOI：
- doi:10.5061/dryad.66hc3
- 摘要：
- by highlighting, for example, the rare biosphere. Currently, high-throughput pyrotag sequencing of PCR-amplified SSU rRNA gene regions is mainly use
Data from: Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies
- 负责人：
- DOI：
- doi:10.5061/dryad.6fm1p
- 摘要：
- by low cost, high throughput and shorter read lengths for example, Illumina. The emergence and development of so called third generation sequencing platforms
Data from: Modular tagging of amplicons using a single PCR for high-throughput sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.22412
- 摘要：
- High-throughput sequencing (HTS) of PCR amplicons is becoming the method of choice to sequence one or several targeted loci for phylogenetic
Data from: Targeted re-sequencing of coding DNA sequences for SNP discovery in non-model species
- 负责人：
- DOI：
- doi:10.5061/dryad.3f4jr01
- 摘要：
- Targeted capture coupled with high throughput sequencing can be used to gain information about nuclear sequence variation at hundreds to thousands
Data from: Modified low-salt CTAB extraction of high-quality DNA from contaminant rich tissues
- 负责人：
- DOI：
- doi:10.5061/dryad.65q20
- 摘要：
- The increasing use of high-throughput sequencing platforms has made the isolation of pure, high molecular weight DNA a primary concern for studies
Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods
- 负责人：
- DOI：
- doi:10.5061/dryad.2p51n
- 摘要：
- The quantification of the biological diversity in environmental samples using high-throughput DNA sequencing is hindered by the PCR bias cause
Data from: DNA metabarcoding reveals that 200-?m-size-fractionated filtering is unable to discriminate between planktonic microbial and lar
- 负责人：
- DOI：
- doi:10.5061/dryad.4m7m1
- 摘要：
- s. High-throughput sequencing is currently revolutionizing their study on an unprecedented scale. However, it is currently unclear whether we can accurately, effectively
Data from: Identification and characterization of sex-associated loci in sockeye salmon using genotyping-by-sequencing and comparison with a se
- 负责人：
- DOI：
- doi:10.5061/dryad.p15br
- 摘要：
- in sockeye salmon (Oncorhynchus nerka). We discovered seven sex-associated loci, developed high-throughput assays for two loci, and tested the utility of these
Data from: Sequencing historical specimens: successful preparation of small specimens with low amounts of degraded DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.31g05
- 摘要：
- . We report successful sample preparation and sequencing for all historical specimens despite our low-input DNA approach. We provide a list of guidelines rela
Data from: High-throughput microsatellite isolation through 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries
- 负责人：
- Malausa, Thibaut
- 关键词：
- Population Genetics - Empirical Genomics/Proteomics Bioinfomatics/Phyloinfomatics Fungi Insects genetic markers
- DOI：
- doi:10.5061/dryad.8297
- 摘要：
- e a high-throughput method for isolating microsatellite markers based on coupling multiplex microsatellite enrichment and Next-Generation Sequencing on 454
