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Data from: A self-preserving, partially biodegradable eDNA filter
- 负责人：
- DOI：
- doi:10.5061/dryad.3s6b300
- 摘要：
- 1. eDNA studies often rely on water filtration in the field and immediate sample preservation to prevent DNA degradation during sample tra
Data from: Sequence capture phylogenomics of historical ethanol-preserved museum specimens: unlocking the rest of the vault
- 负责人：
- 关键词：
- museum collections;degraded DNA;Opiliones;ultraconserved elements;ethanol preservation;phylogenomics
- DOI：
- doi:10.5061/dryad.sh5b962
- 摘要：
- museum specimens with highly degraded DNA, which until recently have been deemed less valuable for genetic work. The successful sequencing of ultra
Data from: Protocols for dry DNA storage and shipment at room temperature
- 负责人：
- DOI：
- doi:10.5061/dryad.6p2h0
- 摘要：
- storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All thr
Data from: Distance, flow, and PCR inhibition: eDNA dynamics in two headwater steams
- 负责人：
- DOI：
- doi:10.5061/dryad.7j3g5
- 摘要：
- the water and DNA leaving the system through degradation, but also on downstream transport. To improve understanding of the dynamics of eDNA concentra
Data from: Compared with conventional PCR assay, qPCR assay greatly improves the detection efficiency of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.1rn8pk0qz
- 摘要：
- owed that qPCR was more sensitive and highly reproducible in detecting such degraded DNA from predator’s gut. The present study provides an example of the us
Data from: Next-generation museum genomics: phylogenetic relationships among palpimanoid spiders using sequence capture techniques (Ara
- 负责人：
- DOI：
- doi:10.5061/dryad.387c58v
- 摘要：
- Historical museum specimens are invaluable for morphological and taxonomic research, but typically the DNA is degraded making traditional sequencing
of environmental DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.87v3v
- 摘要：
- and assessed the degradation rate of eDNA in water. Our primers did not amplify DNA from closely related species, and we found that P. bivittatus DNA
tically admixed
- 负责人：
- DOI：
- doi:10.5061/dryad.g3375
- 摘要：
- we demonstrate that next-generation sequencing can provide high quality data for type material with degraded DNA and therefore can play an important role
Data from: Sequencing historical specimens: successful preparation of small specimens with low amounts of degraded DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.31g05
- 摘要：
- ted to DNA repair, bead handling, reducing adapter dimers, and library amplification. We present these guidelines to facilitate more economical use of valuable DNA
Data from: HyRAD-X, a versatile method combining exome capture and RAD sequencing to extract genomic information from ancient DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.m94f4
- 摘要：
- on RAD-sequencing loci have circumvented this limitation and opened avenues to the study of samples characterized by degraded DNA, such as historical specimens
