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Data from: On the optimal trimming of high-throughput mRNA sequence data
- 负责人:
- DOI:
- doi:10.5061/dryad.7rm34
- 摘要:
- to data quality is required. In particular, because high-throughput sequencing is more error-prone than traditional Sanger sequencing, quality trimming of sequence
Data from: Metazoan mitochondrial gene sequence reference datasets for taxonomic assignment of environmental samples
- 负责人:
- DOI:
- doi:10.5061/dryad.2v00t
- 摘要:
- Mitochondrial-encoded genes are increasingly targeted in studies using high-throughput sequencing approaches for characterizing metazoan communities
Data from: Quantitative PCR primer design affects quantification of dsRNA-mediated gene knockdown
- 负责人:
- DOI:
- doi:10.5061/dryad.28n8d6t
- 摘要:
- of the mRNA transcript sequences should be used for accurate and reliable quantification of silencing efficiency.
Data from: Less is more: extreme genome complexity reduction with ddRAD using Ion Torrent semiconductor technology
- 负责人:
- 关键词:
- Eurasian perch SNP development and validation de novo assembly next-generation sequencing population genomics DNA pooling
- DOI:
- doi:10.5061/dryad.s2405
- 摘要:
- rasian perch. We expect that our ddRAD sequencing protocol for semiconductor sequencing technology will be useful alternative for currently available RAD protocols.
Data from: Deep sequencing of a candidate region harboring the SOX9 gene for the canine XX disorder of sex development
- 负责人:
- Grzemski, Adrian
- DOI:
- doi:10.5061/dryad.65th7
- 摘要:
- and two blocks of an elevated heterozygosity. An initial next-generation sequencing analysis showed an association with two SNPs, but validation in larger cohorts
Transcriptome sequencing of Capra hircus for different tissues.
- 负责人:
- BGI
- DOI:
- doi:10.26036/cnphis0000942
- 摘要:
- hair follicle and secondary hair follicle from three Inner Mongolia cashmere goats and conducted transcriptome sequencing and DGE analysis. Ten RNA pools wer
Data from: Phylogenomics of phrynosomatid lizards: conflicting signals from sequence capture versus restriction site associated DNA sequencing
- 负责人:
- DOI:
- doi:10.5061/dryad.4vt08
- 摘要:
- Sequence capture and restriction site associated DNA sequencing (RADseq) are popular methods for obtaining large numbers of loci
Data from: Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns
- 负责人:
- Bunce, Michael
- DOI:
- doi:10.5061/dryad.8ps58rp2
- 摘要:
- . Here we show that Second Generation, high-throughput sequencing (HTS) of DNA represents an effective means to genetically audit organic ingredients
Data from: Evaluation of the impact of RNA preservation methods of spiders for de novo transcriptome assembly
- 负责人:
- DOI:
- doi:10.5061/dryad.r94k7
- 摘要:
- e obtained. The parameters for sample storage, RNA extraction, library preparation, sequencing, and in silico assembly considered in this work provide
Data from: De novo assembly of a chromosome-level reference genome of red spotted grouper (Epinephelus akaara) using nanopore sequencing and Hi-C
- 负责人:
- DOI:
- doi:10.5061/dryad.4398b9f
- 摘要:
- reference genome of E. akaara by taking advantage of long-read single molecule sequencing and de novo assembly by Oxford Nanopore Technologies (ONT