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Data from: Robust DNA isolation and high-throughput sequencing library construction for herbarium specimens
负责人:
关键词:
herbarium;museomics;grasses;DNA isolation;Schizachyrium scoparium;Andropogoneae;high-throughput sequencing;phylogenomics
DOI:
doi:10.5061/dryad.r8t2m
摘要:
to be adaptable for use in other plant lineages after verification. This protocol can be limited by extremely degraded DNA, where fragments do not exist
Data from: Distance, flow, and PCR inhibition: eDNA dynamics in two headwater steams
负责人:
关键词:
Environmental DNA;Salvelinus fontinalis;stream;Fish;qPCR;eDNA;lotic
DOI:
doi:10.5061/dryad.7j3g5
摘要:
the water and DNA leaving the system through degradation, but also on downstream transport. To improve understanding of the dynamics of eDNA concentrati
Data from: Protocols for dry DNA storage and shipment at room temperature
负责人:
关键词:
DNA preservation;Biomatrica;trehalose;PVA;dry storage
DOI:
doi:10.5061/dryad.6p2h0
摘要:
storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All thr
Data from: Stress response or beneficial temperature acclimation: transcriptomic signatures in Antarctic fish (Pachycara brachycephalum)
负责人:
关键词:
cold-adaptation;Pachycara brachycephalum;microarray;Gene Regulation;chronic thermal exposure;ESTs;cDNA library
DOI:
doi:10.5061/dryad.40rk0
摘要:
was accompanied by the transcriptomic activation of differential protein degradation pathways, from proteasome-based degradation in the cold towards lysosomal protein degradation
Data from: Opening the door to the past: accessing phylogenetic, pathogen, and population data from museum curated bees
负责人:
关键词:
population genetics;ddRAD;Eucera (Peponapis) pruinosa
DOI:
doi:10.5061/dryad.3528sj6
摘要:
l DNA degradation. Specifically, we found that not all samples yielded satisfactory results for molecular studies; however, we did not find a systematic effec
Data from: Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus
负责人:
关键词:
Biotechnology Conservation Biology Fisheries Management Population Genetics - Empirical
DOI:
doi:10.5061/dryad.1797v
摘要:
to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high gene
Data from: Successful carnivore identification with faecal DNA across a fragmented Amazonian landscape
负责人:
关键词:
Habitat Degradation;Mammals;Conservation Biology;Ecological Genetics;Wildlife Management;Panthera onca
DOI:
doi:10.5061/dryad.84220
摘要:
can rapidly degrade DNA. Despite these potential problems, we demonstrate successful DNA amplification and sequencing for faeces of carnivores collected
Data from: Phylogeography and support vector machine classification of colour variation in panther chameleons
负责人:
关键词:
Support Vector Machine classification;supervised learning;phylogeography;panther chameleon;colour patterns;Furcifer pardalis
DOI:
doi:10.5061/dryad.74b7h
摘要:
Lizards and snakes exhibit colour variation of adaptive value for thermoregulation, camouflage, predator avoidance, sexual selection and speciati
Data from: Sequencing historical specimens: successful preparation of small specimens with low amounts of degraded DNA
负责人:
关键词:
Museum specimens;Bembidion;insects;Coleoptera;high-throughput sequencing;Insecta;natural history collections;Carabidae;Sample Preparation;type specimens;Lionepha
DOI:
doi:10.5061/dryad.31g05
摘要:
ted to DNA repair, bead handling, reducing adapter dimers, and library amplification. We present these guidelines to facilitate more economical use of valuable DNA
Data from: From the field to the lab: best practices for field preservation of bat specimens for molecular analyses
负责人:
关键词:
Field protocols;Wing punches;DNA preservation;DMSO;Affordable field preservation solutions;ETOH;Silica gel;Field preservation;Mammals;Chiroptera
DOI:
doi:10.5061/dryad.3d727
摘要:
s never been directly tested, even though these media may influence DNA degradation under field conditions. We analyzed DNA yield from buccal swabs

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