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Data from: Robust DNA isolation and high-throughput sequencing library construction for herbarium specimens
- 负责人:
- DOI:
- doi:10.5061/dryad.r8t2m
- 摘要:
- to be adaptable for use in other plant lineages after verification. This protocol can be limited by extremely degraded DNA, where fragments do not exist
Data from: Distance, flow, and PCR inhibition: eDNA dynamics in two headwater steams
- 负责人:
- DOI:
- doi:10.5061/dryad.7j3g5
- 摘要:
- the water and DNA leaving the system through degradation, but also on downstream transport. To improve understanding of the dynamics of eDNA concentrati
Data from: Protocols for dry DNA storage and shipment at room temperature
- 负责人:
- DOI:
- doi:10.5061/dryad.6p2h0
- 摘要:
- storage at room temperature, dry storage provides an additional layer of security for frozen samples, protecting them from degradation in the event of freezer failure. All thr
Data from: Stress response or beneficial temperature acclimation: transcriptomic signatures in Antarctic fish (Pachycara brachycephalum)
- 负责人:
- DOI:
- doi:10.5061/dryad.40rk0
- 摘要:
- was accompanied by the transcriptomic activation of differential protein degradation pathways, from proteasome-based degradation in the cold towards lysosomal protein degradation
Data from: Opening the door to the past: accessing phylogenetic, pathogen, and population data from museum curated bees
- 负责人:
- DOI:
- doi:10.5061/dryad.3528sj6
- 摘要:
- l DNA degradation. Specifically, we found that not all samples yielded satisfactory results for molecular studies; however, we did not find a systematic effec
Data from: Population differentiation determined from putative neutral and divergent adaptive genetic markers in Eulachon (Thaleichthys pacificus
- 负责人:
- DOI:
- doi:10.5061/dryad.1797v
- 摘要:
- to the Columbia River were examined by restriction-site-associated DNA (RAD) sequencing to elucidate patterns of neutral and adaptive variation in this high gene
Data from: Successful carnivore identification with faecal DNA across a fragmented Amazonian landscape
- 负责人:
- DOI:
- doi:10.5061/dryad.84220
- 摘要:
- can rapidly degrade DNA. Despite these potential problems, we demonstrate successful DNA amplification and sequencing for faeces of carnivores collected
Data from: Phylogeography and support vector machine classification of colour variation in panther chameleons
- 负责人:
- DOI:
- doi:10.5061/dryad.74b7h
- 摘要:
- Lizards and snakes exhibit colour variation of adaptive value for thermoregulation, camouflage, predator avoidance, sexual selection and speciati
Data from: Sequencing historical specimens: successful preparation of small specimens with low amounts of degraded DNA
- 负责人:
- DOI:
- doi:10.5061/dryad.31g05
- 摘要:
- ted to DNA repair, bead handling, reducing adapter dimers, and library amplification. We present these guidelines to facilitate more economical use of valuable DNA
Data from: From the field to the lab: best practices for field preservation of bat specimens for molecular analyses
- 负责人:
- DOI:
- doi:10.5061/dryad.3d727
- 摘要:
- s never been directly tested, even though these media may influence DNA degradation under field conditions. We analyzed DNA yield from buccal swabs