筛选
科学数据
统计数据
共检索到104条 ,权限内显示50条;
Data from: Ligation of glycophorin A generates reactive oxygen species leading to decreased red blood cell function
- 负责人：
- DOI：
- doi:10.5061/dryad.f862c
- 摘要：
- Acute, inflammatory conditions associated with dysregulated complement activation are characterized by significant increases in blood concentration of reactive oxygen species (ROS) and ATP. The mechanisms by which these molecules arise are not fully understood. In this study, using luminometric- and fluorescence-based methods, we show that ligation of glycophorin A (GPA) on human red blood cells (RBCs) results in a 2.1-fold, NADPH-oxidase-dependent increase in intracellular ROS that, in turn, trigger multiple downstream cascades leading to caspase-3 activation, ATP release, and increased band 3 phosphorylation. Functionally, using 2D microchannels to assess membrane deformability, GPS-ligated RBCs travel 33% slower than control RBCs, and lipid mobility was hindered by 10% using fluorescence recovery after photobleaching (FRAP). These outcomes were preventable by pretreating RBCs with cell-permeable ROS scavenger glutathione monoethyl ester (GSH-ME). Our results obtained in vitro using anti-GPA antibodies were validated using complement-altered RBCs isolated from control and septic patients. Our results suggest that during inflammatory conditions, circulating RBCs significantly contribute to capillary flow dysfunctions, and constitute an important but overlooked source of intravascular ROS and ATP, both critical mediators responsible for endothelial cell activation, microcirculation impairment, platelet activation, as well as long-term dysregulated adaptive and innate immune responses.
Data from: Incidence of influenza A(H3N2) virus infections in Hong Kong in a longitudinal sero-epidemiological study, 2009-2015
- 负责人：
- DOI：
- doi:10.5061/dryad.7c93n5s
- 摘要：
- ) virus, and samples from 2013-15 against the A/Victoria/361/2011(H3N2) virus using the hemagglutination inhibition (HAI) assay. We estimated
Data from: A keystone mutualism underpins resilience of a coastal ecosystem to drought
- 负责人：
- DOI：
- doi:10.5061/dryad.d875g
- 摘要：
- Droughts are increasing in severity and frequency, yet the mechanisms that strengthen ecosystem resilience to this stress remain poorly understood. Here, we test whether positive interactions in the form of a mutualism between mussels and dominant cordgrass in salt marshes enhance ecosystem resistance to and recovery from drought. Surveys spanning 250?km of southeastern US coastline reveal spatially dispersed mussel mounds increased cordgrass survival during severe drought by 5- to 25-times. Surveys and mussel addition experiments indicate this positive effect of mussels on cordgrass was due to mounds enhancing water storage and reducing soil salinity stress. Observations and models then demonstrate that surviving cordgrass patches associated with mussels function as nuclei for vegetative re-growth and, despite covering only 0.1–12% of die-offs, markedly shorten marsh recovery periods. These results indicate that mutualisms, in supporting stress-resistant patches, can play a disproportionately large, keystone role in enhancing ecosystem resilience to climatic extremes.
Data from: Inferring HIV-1 transmission networks and sources of epidemic spread in Africa with deep-sequence phylogenetic analysis
- 负责人：
- DOI：
- doi:10.5061/dryad.7h46hg2
- 摘要：
- To prevent new infections with human immunodeficiency virus type 1 (HIV-1) in sub-Saharan Africa, UNAIDS recommends targeting interventions to populations that are at high risk of acquiring and passing on the virus. Yet it is often unclear who and where these ‘source’ populations are. Here we demonstrate how viral deep-sequencing can be used to reconstruct HIV-1 transmission networks and to infer the direction of transmission in these networks. We are able to deep-sequence virus from a large population-based sample of infected individuals in Rakai District, Uganda, reconstruct partial transmission networks, and infer the direction of transmission within them at an estimated error rate of 16.3% [8.8–28.3%]. With this error rate, deep-sequence phylogenetics cannot be used against individuals in legal contexts, but is sufficiently low for population-level inferences into the sources of epidemic spread. The technique presents new opportunities for characterizing source populations and for targeting of HIV-1 prevention interventions in Africa.
Data from: The natural history of the South Hills crossbill in relation to its impending extinction
- 负责人：
- DOI：
- doi:10.5061/dryad.cm586
- 摘要：
- ween 2003 and 2011. This is an example of a novel trophic mismatch between a consumer and its resource caused by a shift in the phenology of the resource arising from
GBIF Occurrence Download
- 负责人：
- DOI：
- doi:10.15468/dl.epccec
- 摘要：
- of the Atlantic (South Benin), 2011. 38 records from Census of the flora of Benin. 1 records from Census of secondary forest of Itchèdè-Toffo (Southern Benin
GBIF Occurrence Download
- 负责人：
- DOI：
- doi:10.15468/dl.bl4xhj
- 摘要：
- in the framework of BID National project BID-AF2015-0065-NAC and funded by EU. 6 records from Census of medicinal plants of Benin.. 9 records from
GBIF Occurrence Download
- 负责人：
- DOI：
- doi:10.15468/dl.ydbxsp
- 摘要：
- Survey of Ireland 2006 & 2007. 115 records from NE Scotland Wildcat records 1800-2011. 1287 records from Atlas of Mammals in Ireland 2010-2015. 90
GBIF Occurrence Download
- 负责人：
- DOI：
- doi:10.15468/dl.r9udhd
- 摘要：
- mobilized in the framework of BID National project BID-AF2015-0065-NAC and funded by EU. 9 records from Geographically tagged INSDC sequences. 27 records
GBIF Occurrence Download
- 负责人：
- DOI：
- doi:10.15468/dl.pfsruq
- 摘要：
- of BID National project BID-AF2015-0065-NAC and funded by EU. 9 records from Census of medicinal and agroforestry plants of Benin. 3 records from
