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Data from: SSR_pipeline: a bioinformatic infrastructure for identifying microsatellites from paired-end Illumina high-throughput DNA sequencing data
负责人:
关键词:
microsatellite discovery;Next Generation Sequencing;Ephydra packardi;Python
DOI:
doi:10.5061/dryad.n65k2
摘要:
DNA sequencing run. All modules from SSR_pipeline are implemented in the Python programming language and can therefore be used from nearly any computer
Data from: Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison
负责人:
关键词:
Formalin-fixed paraffin-embedded;Colorectal cancer;Targeted next-generation sequencing;RAS mutations;Ion Torrent Personal Genome Machine
DOI:
doi:10.5061/dryad.3447g
摘要:
Objective: To validate the targeted next-generation sequencing (NGS) platform-Ion Torrent PGM for KRAS exon 2 and expanded RAS mutations detecti
Data from: Next-generation sequencing to inventory taxonomic diversity in eukaryotic communities: a test for freshwater diatoms
负责人:
关键词:
DNA Barcoding;metagenomics;Bacillariophyta;diatoms;Algae;Community Ecology
DOI:
doi:10.5061/dryad.370nq
摘要:
The recent emergence of barcoding approaches coupled to those of Next Generation Sequencing (NGS) have raised new perspectives for studying
Data from: Impacts of degraded DNA on restriction enzyme associated DNA sequencing (RADSeq)
负责人:
关键词:
SNP;Reduced Representation Libraries;Coregonus clupeaformis;ddRADseq
DOI:
doi:10.5061/dryad.mf1ck
摘要:
-generation DNA sequencing (RADSeq, GBS) is unknown. We experimentally examined the effects of in situDNA degradation on data generation for a modified double
Data from: Cost-efficient high throughput capture of museum arthropod specimen DNA using PCR-generated baits
负责人:
关键词:
Miridae;insects;host plant;Phylinae;phylogeny
DOI:
doi:10.5061/dryad.8004512
摘要:
h as arthropods. Next generation sequencing techniques allow for sequencing of short DNA fragments contained in preserved specimens >20 years old, but approaches
by next-generation sequencing
负责人:
关键词:
Natural Selection and Contemporary Evolution;Transcriptomics;Coregonus clupeaformis;molecular evolution;Fish;speciation
DOI:
doi:10.5061/dryad.1924
摘要:
Gene expression divergence is one of the mechanisms thought to be involved in the emergence of incipient species. Next-generation sequencing has bec
Data from: Tissue storage and primer selection influence pyrosequencing-based inferences of diversity and community composition of endolichenic
负责人:
关键词:
moss;diversity;Peltigera praetextata;Brachythecium fendleri;454 amplicon pyrosequencing;ITSrDNA;lichen;Pinus leiophylla var. chihuahuana;mycobiont
DOI:
doi:10.5061/dryad.8271f
摘要:
Next-generation sequencing technologies have provided unprecedented insights into fungal diversity and ecology. However, intrinsic biase
Data from: Genomic repeat abundances contain phylogenetic signal
负责人:
关键词:
repetitive DNA;Nicotiana;Next-generation sequencing;genomics;phylogenetics;continuous characters;molecular systematics
DOI:
doi:10.5061/dryad.vn0gc
摘要:
A large proportion of genomic information, particularly repetitive elements, is usually ignored when researchers are using next-generation sequencing
Data from: Unveiling the diet of elusive rainforest herbivores in next generation sequencing era? The tapir as a case study
负责人:
关键词:
ungulate;Tapirus terrestris;Neotropical;faecal DNA;non-invasive;Next-generation sequencing;meta barcoding;diet;indirect approach;herbivore;tapir;browse
DOI:
doi:10.5061/dryad.8h7k1
摘要:
s, referred to as DNA metabarcoding, to investigate the diet of the largest neotropical herbivore, the lowland tapir. Sequencing was performed on plant DNA extra
Data from: Tag jumps illuminated – reducing sequence-to-sample misidentifications in metabarcoding studies
负责人:
关键词:
Environmental DNA;Metabarcoding;Tag jumping;Diversity assessment;Second generation sequencing;Chimeras
DOI:
doi:10.5061/dryad.64687
摘要:
Metabarcoding of environmental samples on second-generation sequencing platforms has rapidly become a valuable tool for ecological studies

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