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adaptive sampling of environmental DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.1fp259b
- 摘要：
- , and amplification), future studies should collect 20 water samples per site, conduct at least 2 extractions per sample, and conduct 5 qPCR replicates per extraction
Data from: A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples
- 负责人：
- DOI：
- doi:10.5061/dryad.92bh000
- 摘要：
- membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract
Data from: Extracting DNA from ‘jaws’: high yield and quality from archived tiger shark (Galeocerdo cuvier) skeletal material
- 负责人：
- DOI：
- doi:10.5061/dryad.8080c
- 摘要：
- e to evaluate and optimize extraction methods, which may be crucial for downstream applications. Here, we assessed and optimized the usefulness of abundant
Data from: Finding flies in the mushroom soup: host specificity of fungus-associated communities revisited with a novel molecular method
- 负责人：
- DOI：
- doi:10.5061/dryad.tf56fm1
- 摘要：
- a pipeline applicable for extracting amplifiable total DNA from soft fungal samples of any size. Our protocol purifies DNA in two sequential steps: (1) initi
Data from: A new plant virus discovered by immunocapture of double stranded RNA; assessment of a novel approach for viral metagenomics studies
- 负责人：
- Blouin, Arnaud G.
- 关键词：
- Metagenomics antibodies dsRNA NGS virus
- DOI：
- doi:10.5061/dryad.0bg04
- 摘要：
- plant extracts using anti-dsRNA monoclonal antibodies in a pull-down assay. The extracted dsRNA can be amplified by reverse transcriptase–polymerase chain reacti
Data from: Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol
- 负责人：
- Zarzoso-Lacoste, Diane
- 关键词：
- Invasive rats Diet Analysis PCR-based method Extraction protocol performances A260/A280 absorbance ratio Group-specific primer pairs
- DOI：
- doi:10.5061/dryad.8c134
- 摘要：
- in the predator food bolus and have proved themselves efficient, with high accuracy. When studying complex diet samples, the extraction of total DNA is a criti
Data from: Development and validation of rapid environmental DNA (eDNA) detection methods for bog turtle (Glyptemys muhlenbergii)
- 负责人：
- DOI：
- doi:10.5061/dryad.6n7675r
- 摘要：
- DNA extraction methods, limit false negative results due to qPCR inhibition, and measure total DNA recovery from each sample. Using the internal control, we found that DNA
Data from: Detection of the endangered European weather loach (Misgurnus fossilis) via water and sediment samples: testing multiple eDNA workflows.
- 负责人：
- DOI：
- doi:10.5061/dryad.4xgxd256h
- 摘要：
- combinations for long-term sample preservation, DNA capture and DNA extraction. Additionally, we analyzed the DNA yield of samples from a ditch with a natura
Data from: Tracking earthworm communities from soil DNA
- 负责人：
- Taberlet, Pierre
- DOI：
- doi:10.5061/dryad.3mv8v434
- 摘要：
- of ecosystem health. To date, earthworm diversity has been investigated through conventional extraction methods such as handsorting, soil washing or the applicati
Data from: Toward a standardised protocol for the stable isotope analysis of scleractinian corals
- 负责人：
- DOI：
- doi:10.5061/dryad.xsj3tx99s
- 摘要：
- (Stylophora pistillata and Porites lutea) were divided into subsamples to test the effects of the drying method, lipid extraction
