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Data from: A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples
- 负责人：
- DOI：
- doi:10.5061/dryad.92bh000
- 摘要：
- ly. High-performance liquid chromatography (HPLC) was employed to confirm the performance of the strip. The strip test only took less than 10 minutes, and thus provides a repaid method
Data from: Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols
- 负责人：
- DOI：
- doi:10.5061/dryad.8fb46m5
- 摘要：
- high performance liquid chromatography (HPLC) data with ~100,000 single nucleotide polymorphisms (SNPs) from two diverse apple populations. We found that polyphenols can vary in concentration
Data from: Fecal cortisol metabolites to assess stress in wildlife: evaluation of a field method in free ranging chamois
- 负责人：
- DOI：
- doi:10.5061/dryad.5n60v
- 摘要：
- , a high-performance liquid chromatography (HPLC) was performed. Sex-specific differences in metabolite excretion were evaluated. Effects of storage length and temperature on FCM were tested
Data from: Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation.
- 负责人：
- DOI：
- doi:10.5061/dryad.m5c7m
- 摘要：
- and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37
Data from: Biotransformation of chlorothalonil by strain Stenotrophomonas acidaminiphila BJ1 isolated from farmland soil
- 负责人：
- DOI：
- doi:10.5061/dryad.v15fc6b
- 摘要：
- e detected and identified based on HPLC-MS analysis, which suggests that the strain BJ1 metabolized chlorothalonil through the hydroxylation of chloro-group a
Data from: Exhaustive extraction of cyclopeptides from Amanita phalloides: guidelines for working with complex mixtures of secondary metabolites
- 负责人：
- DOI：
- doi:10.5061/dryad.47d7wm398
- 摘要：
- Understanding plant-insect interactions is an active area of research in both ecology and evolution. Much attention has been focused the impact of secondary metabolites in the host plant or fungi on these interactions. Plants and fungi contain a variety of biologically active compounds, and the secondary metabolite profile can vary significantly between individual samples. However, many experiments characterize the biological effects of only a single secondary metabolite or a subset of these compounds.
- Here, we develop an exhaustive extraction protocol using an accelerated solvent extraction protocol to recover the complete suite of secondary metabolites found in Amanita phalloides (death cap mushrooms) and compare its efficacy to the ‘Classic’ extraction method used in earlier works.
- We demonstrate that our extraction protocol recovers the full suite of secondary metabolites in A. phalloides unlike the ‘Classic’ method that favors polar secondary metabolites.
- Based on these findings, we provide recommendations for how to optimize protocols to ensure exhaustive extracts and also the best practices when using natural extracts in ecological experiments.
Data from: Biogenic amine modulation of honey bee sociability and nestmate affiliation
- 负责人：
- DOI：
- doi:10.5061/dryad.n720m21
- 摘要：
- . Octopamine treatment blocked social interaction with other bees, and serotonin increased the likelihood of social interactions. HPLC measurements showed tha
Data from: Amplicon-pyrosequencing based detection of compositional shifts in bryophyte-associated fungal communities along an elevation gradient.
- 负责人：
- DOI：
- doi:10.5061/dryad.c2hf0
- 摘要：
- . HPLC assays of ergosterol (fungal biomass) and amplicon pyrosequencing of the ITS2 region of rDNA were used to investigate how the fungal communities
Data from: Macroevolution of leaf defenses and secondary metabolites across the genus Helianthus
- 负责人：
- DOI：
- doi:10.5061/dryad.5hq56
- 摘要：
- Leaf defenses are widely recognized as key adaptations and drivers of plant evolution. Across environmentally diverse habitats, the macroevolution of leaf defenses can be predicted by the univariate trade-off model, which predicts that defenses are functionally redundant and thus trade off, and the resource availability hypothesis, which predicts that defense investment is determined by inherent growth rate and that higher defense will evolve in lower resource environments. Here, we examined the evolution of leaf physical and chemical defenses and secondary metabolites in relation to environmental characteristics and leaf economic strategy across 28 species of Helianthus (the sunflowers). Using a phylogenetic comparative approach, we found few evolutionary trade-offs among defenses and no evidence for defense syndromes. We also found that leaf defenses are strongly related to leaf economic strategy, with higher defense in more resource-conservative species, although there is little support for the evolution of higher defense in low-resource habitats. A wide variety of physical and chemical defenses predict resistance to different insect herbivores, fungal pathogens, and a parasitic plant, suggesting that most sunflower defenses are not redundant in function and that wild Helianthus represents a rich source of variation for the improvement of crop sunflower.
Data from: Hyaluronan and N-ERC/mesothelin as key biomarkers in a specific two-step model to predict pleural malignant mesothelioma
- 负责人：
- DOI：
- doi:10.5061/dryad.h122b
- 摘要：
- centre. Hyaluronan, N-ERC/mesothelin, C-ERC/mesothelin, osteopontin, syndecan-1, syndecan-2, and thioredoxin were measured using ELISA and HPLC
