Data from: From benchtop to desktop: important considerations when designing amplicon sequencing workflows

负责人：

关键词：

Amplicon Sequencing;high throughput sequencing;DNA metabarcoding;Second generation sequencing;Next-generation sequencing

DOI：

doi:10.5061/dryad.2qf0t

摘要：

involving multiple PCR steps. This paper redresses this imbalance by focusing on aspects pertaining to the benchtop within typical amplicon workflows: sample

Data from: Extreme copy number variation at a tRNA ligase gene affecting phenology and fitness in yellow monkeyflowers

负责人：

关键词：

Life History Evolution;quantitative genetics;Holocene;Contemporary Evolution;Ecological Genetics;Angiosperms;Mimulus guttatus;Adaptation

DOI：

doi:10.5061/dryad.m1j24tf

摘要：

250-300 copies of RLG1a totaling ~5.7Mb, equivalent to 20-40% of a chromosome. Mendelian segregation of diagnostic alleles and qPCR-based copy counts