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Data from: Validation of COI metabarcoding primers for terrestrial arthropods
负责人:
关键词:
DNA metabarcoding;Primer bias;Degeneracy;insects;biodiversity
DOI:
doi:10.5061/dryad.249rk92
摘要:
Metabarcoding can rapidly determine the species composition of bulk samples and thus aids biodiversity and ecosystem assessment. Howeve
Data from: Environmental DNA reveals quantitative patterns of fish biodiversity in large rivers despite its downstream transportation
负责人:
关键词:
eDNA metabarcoding;detection distance;biodiversity patterns;Actinopteri
DOI:
doi:10.5061/dryad.t4n42rr
摘要:
nks). Using an eDNA metabarcoding approach, we regularly sampled 500 km of a large river (Rh?ne River). Comparisons with long-term electrofishing surveys
Data from: The choice of universal primers and the characteristics of the species mixture determines when DNA metabarcoding can be quantitative.
负责人:
关键词:
Environmental DNA;insects;in silico PCR;Primer bias;COI;Diet Analysis
DOI:
doi:10.5061/dryad.q2r3b1f
摘要:
DNA metabarcoding is a technique used to survey biodiversity in many ecological settings, but there are doubts about whether it can provide
Data from: Tag jumps illuminated – reducing sequence-to-sample misidentifications in metabarcoding studies
负责人:
关键词:
Environmental DNA;Metabarcoding;Tag jumping;Diversity assessment;Second generation sequencing;Chimeras
DOI:
doi:10.5061/dryad.64687
摘要:
Metabarcoding of environmental samples on second-generation sequencing platforms has rapidly become a valuable tool for ecological studi
Data from: Ecosystem biomonitoring with eDNA: metabarcoding across the tree of life in a tropical marine environment
负责人:
关键词:
eukaryotes;ecosystem based management;biodiversity;prokaryotes;Environmental DNA;shotgun sequencing
DOI:
doi:10.5061/dryad.qq11c
摘要:
ven the sensitivity and low cost of eDNA metabarcoding we advocate this approach be rapidly integrated into biomonitoring programs.
Data from: A from-benchtop-to-desktop workflow for validating HTS data and for taxonomic identification in diet metabarcoding studies
负责人:
关键词:
Metabarcoding;Zingel asper;taxonomic assignment;Diet studies;Pomatoschistus microps;COI;HTS data filtering
DOI:
doi:10.5061/dryad.f40v5
摘要:
The main objective of this work was to develop and validate a robust and reliable ‘from benchtop-to-desktop’ metabarcoding workflow to investigate
Data from: ITS all right mama: Investigating the formation of chimeric sequences in the ITS2 region by DNA metabarcoding analyses of fungal mock
负责人:
关键词:
DNA metabarcoding;Chimeric sequences;PCR bias;UCHIME;microbial ecology
DOI:
doi:10.5061/dryad.n4cv3
摘要:
The formation of chimeric sequences can create significant methodological bias in PCR-based DNA metabarcoding analyses. During mixed-template
Data from: Metabarcoding using multiplexed markers increases species detection in complex zooplankton communities
负责人:
关键词:
Metabarcoding;Zooplankton;multigene;multiple primer pairs;COI;18S;Zooplankton
DOI:
doi:10.5061/dryad.m83jc20
摘要:
Metabarcoding combines DNA barcoding with high-throughput sequencing, often using one genetic marker to understand complex and taxonomically di
Data from: Effects of sampling effort on biodiversity patterns estimated from environmental DNA metabarcoding surveys
负责人:
关键词:
Metazoan;non-indigenous;port;eDNA;Holocene
DOI:
doi:10.5061/dryad.40782nd
摘要:
Environmental DNA (eDNA) metabarcoding can greatly enhance our understanding of global biodiversity and our ability to detect rare or cryptic species

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