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Data from: Attack of the PCR clones: rates of clonality have little effect on RAD-seq genotype calls
- 负责人：
- DOI：
- doi:10.5061/dryad.3mq4631
- 摘要：
- , investigate the mechanisms that lead to genotype call changes, and test if clones bias heterozygosity estimates. Our RAD-seq datasets contained 30 – 60% PCR
Data from: Long-range PCR allows sequencing of mitochondrial genomes from environmental DNA
- 负责人：
- DOI：
- doi:10.5061/dryad.q5gg0
- 摘要：
- range of ray-finned fishes (Class: Actinopterygii). We tested the primer pair using long-range PCR and Illumina sequencing in vitro on a mock community
Data from: Can long-range PCR be used to amplify genetically divergent mitochondrial genomes for comparative phylogenetics? A case study
- 负责人：
- DOI：
- doi:10.5061/dryad.8dd3n
- 摘要：
- e we assess the effectiveness of a wide range of targeted long-range PCR strategies, encapsulating single and dual fragment primer design approaches to provide
Data from: Residual eDNA detection sensitivity assessed by quantitative real-time PCR in a river ecosystem
- 负责人：
- DOI：
- doi:10.5061/dryad.5rf92
- 摘要：
- eDNA detection sensitivity using quantitative real-time polymerase chain reaction (qRT-PCR), in a flowing, uncontrolled river after the eDNA source
Data from: Optimizing methods for PCR-based analysis of predation
- 负责人：
- DOI：
- doi:10.5061/dryad.8947
- 摘要：
- ), we found that only in the longest PCR product, a marked decrease in prey detection success occurred. Lowering maximum annealing temperatures by 4 °C resulte
Data from: Diagnostic gene expression biomarkers of coral thermal stress
- 负责人：
- 关键词：
- qPCR;Porites astreoides;cellular stress response;double-gene assay;cellular homeostasis response
- DOI：
- doi:10.5061/dryad.1h0n1
- 摘要：
- erested in linking organism physiology with large-scale climatic conditions. Here, we assessed the ability of quantitative PCR (qPCR) based gene expression biomarkers
Data from: High herpesvirus diversity in wild rodent and shrew species in central Africa
- 负责人：
- Christian Lange
- 关键词：
- DOI：
- doi:10.5061/dryad.7880kj7
- 摘要：
- e sampled and tested by PCR for the presence of herpesvirus DNA. Methods: A broad range PCRs targeting either the Polymerase or the terminase gene were use
Data from: Advanced characterization of DNA molecules in rAAV vector preparations by single-stranded virus next-generation sequencing
- 负责人：
- DOI：
- doi:10.5061/dryad.fs4cp
- 摘要：
- with qPCR results and showed a quasi-random distribution of contaminants originating from the packaging cells genome. Finally, we found single-nucleotide
Data from: High Rates of Asymptomatic, Sub-microscopic Plasmodium vivax Infection and Disappearing Plasmodium falciparum Malaria in an Area of Low
- 负责人：
- DOI：
- doi:10.5061/dryad.545tv
- 摘要：
- by quantitative PCR (qPCR) and light microscopy (LM). Presence of gametocytes was determined by reverse transcription quantitative PCR (RT-qPCR). Results: By qPCR, 468
Data from: Deep sequencing of the olfactory epithelium reveals specific chemosensory receptors are expressed at sexual maturity in the European eel Anguilla anguilla
- 负责人：
- DOI：
- doi:10.5061/dryad.qp578
- 摘要：
- Vertebrate genomes encode a diversity of G protein-coupled receptors (GPCR) that belong to large gene families and are used by olfactory system
