Data from: Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods

负责人：

关键词：

Phyllocnistis citrella;PCR cycles;Orius majusculus;Entomobrya sp.;PCR bias;Theridion sp.;annealing temperature;Macrolophus melanotoma;Orius laevigatus;Philodromus cespitum;Adalia decempunctata;Trichopsocus clarus;insects;Episyrphus balteatus;Ion Torrent;Aphidius colemani;Aphis spiraecola

DOI：

doi:10.5061/dryad.2p51n

摘要：

DNA, so often a predator-specific blocking oligonucleotide is used to alleviate the problem. However, specific blocking oligonucleotides could coblock

Data from: Carnivore diet analysis based on next-generation sequencing: application to the leopard cat (Prionailurus bengalensis) in Pakistan

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关键词：

Mus musculus;Mitochondrial DNA;ribosomal DNA;Columba livia;Crocidura pullata;Lepus capensis;Hyperacrius wynnei;Pucrasia macrolopha;Corvus macrorhynchos;Timaliidae;Dendrocopos sp.;Eoglaucomys fimbriatus;Dryomys nitedula;Prionailurus bengalensis;Paa vicina;Siluriformes;DNA metabarcoding;species identification;Lophura leucomelanos;Alectoris Chukar;Apodemus rusiges;blocking primer;Rattus rattus;Gallus gallus

DOI：

doi:10.5061/dryad.443t4m1q

摘要：

bp of the mitochondrial 12S rRNA gene, with and without a blocking oligonucleotide specific to the predator sequence. The amplification products wer

Data from: Forest without prey: livestock sustain a common leopard population in Pakistan

负责人：

关键词：

blocking oligonucleotide DNA metabarcoding human-carnivore conflict next generation sequencing

DOI：

doi:10.5061/dryad.87rm1

摘要：

gate this conflict: (1) introducing incentives for increased acceptance of leopards among local communities in the vicinity of the protected area and (2) increasing