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Data from: Pitfalls during in silico prediction of primer specificity for eDNA surveillance
- 负责人：
- 关键词：
- DOI：
- doi:10.5061/dryad.41ns1rnb7
- 摘要：
- qPCR analyses, with 90% of the reactions tested with non-target templates. Primer pairs predicted by Primer-BLAST to be specific rarely showed suc
Data from: Non-specific amplification compromises environmental DNA metabarcoding with COI
- 负责人：
- DOI：
- doi:10.5061/dryad.b8f6s44
- 摘要：
- ee COI primer pairs and one 12S pair for their specificity, reproducibility, and congruence with independent datasets derived from traditional
Data from: Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity
- 负责人：
- DOI：
- doi:10.5061/dryad.p3ks4
- 摘要：
- of Plantae and Fungi from GenBank, we designed new universal and plant-specific PCR primers for amplifying the whole ITS region and a part of it (ITS1 or ITS2
Data from: Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction pro
- 负责人：
- Zarzoso-Lacoste, Diane
- 关键词：
- Invasive rats Diet Analysis PCR-based method Extraction protocol performances A260/A280 absorbance ratio Group-specific primer pairs
- DOI：
- doi:10.5061/dryad.8c134
- 摘要：
- selected to be use as group-specific primer pairs in PCR protocols. The performances of four DNA extraction protocols (QIAamp DNA stool mini kit, DNeasy
Data from: Identification of chloroplast genome loci suitable for high-resolution phylogeographic studies of Colocasia esculenta (L.) Schott (Araceae
- 负责人：
- DOI：
- doi:10.5061/dryad.388ds
- 摘要：
- ats in the chloroplast genome of taro (Colocasia esculenta), we designed 30 primer pairs to amplify and sequence polymorphic loci. The primers have been tested in a range
Data from: Allelic variation of BnaC.TT2.a and its association with seed coat color and fatty acids in rapeseed (Brassica napus L.)
- 负责人：
- DOI：
- doi:10.5061/dryad.52t20
- 摘要：
- . A single copy of BnaC.TT2.a for single nucleotide polymorphism (SNP) assay was cloned by a pair of previously reported specific primers. From the analysis
an species of Bartsia l. (Orobanchaceae)
- 负责人：
- DOI：
- doi:10.5061/dryad.fh592
- 摘要：
- target regions across 48 samples, incorporating sample-specific barcodes and HTS adapters (2,304 unique amplicons per Access Array). The final pro
Data from: Transcriptome-wide mining, characterization, and development of microsatellite markers in Lychnis kiusiana (Caryophyllaceae)
- 负责人：
- DOI：
- doi:10.5061/dryad.rc47tt4
- 摘要：
- tively, and locus-specific FIS estimates ranged from -0.116 to 0.290. Eleven of the 25 primer pairs were successfully amplified in three additional
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