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Data from: More than skin and bones: comparing extraction methods and alternative sources of DNA from avian museum specimens
负责人:
关键词:
toe pad;historical DNA;DNA extraction;avian museum specimens;skin punch;phenol-chloroform;natural history collections
DOI:
doi:10.5061/dryad.0nj71g0
摘要:
of museum genomics grows, so does the need for extraction methods that maximize DNA yields. For avian museum specimens, the established method of extracting
Data from: Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish
负责人:
关键词:
Conservation Biology Environmental DNA Fish Fisheries Management Invasive Species
DOI:
doi:10.5061/dryad.2f681
摘要:
Few studies have examined capture and extraction methods for environmental DNA (eDNA) to identify techniques optimal for detection and quantificati
Data from: Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA
负责人:
关键词:
multi-taxa;DNA extraction protocol;Soil biodiversity;Eukaryota;DNA metabarcoding;Tropical Forest;bacteria;present;Holocene;Viridiplantae
DOI:
doi:10.5061/dryad.1qt12
摘要:
with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded mor
Data from: Modified low-salt CTAB extraction of high-quality DNA from contaminant rich tissues
负责人:
关键词:
Molluscs;DNA extraction;high-throughput sequencing;Crassostrea virginica;genomics;Invertebrates;Polysaccharide
DOI:
doi:10.5061/dryad.65q20
摘要:
provides a cost-effective, scalable, alternative DNA extraction method that can be easily optimized and adapted for sequencing applications in other
Data from: Choice of capture and extraction methods affect detection of freshwater biodiversity from environmental DNA
负责人:
关键词:
metazoan eukaryote;molecular protocols;eubacteria;Freshwater;cytochrome c oxidase I;eDNA;16S;targeted species detection
DOI:
doi:10.5061/dryad.7q19c
摘要:
Environmental DNA (eDNA) is used to detect biodiversity by the capture, extraction, and identification of DNA shed to the environment. However, eDNA
Data from: A cost-efficient and simple protocol to enrich prey DNA from extractions of predatory arthropods for large-scale gut content analysis
负责人:
关键词:
Hololena adnexa
DOI:
doi:10.5061/dryad.311mm
摘要:
predator extracts by selectively removing high molecular weight DNA and by extracting from the midgut and hindgut only. A comparison of our result
Data from: Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae
负责人:
关键词:
Environmental DNA;Chironomidae;DNA Barcoding;insects;species identification;Community Ecology;Invertebrates;COI;Ecological Genetics;Aquatic Macroinvertebrates
DOI:
doi:10.5061/dryad.tf6r4
摘要:
e, we compare the relative effectiveness of five different DNA extraction protocols and direct PCR in isolation of DNA from chironomid pupal exuviae. Chironomidae
Data from: Which specimens from a museum collection will yield DNA barcodes? A time series study of spiders in alcohol
负责人:
关键词:
DNA extraction;DNA preservation;DNA concentration;museum;independent contrasts;DNA degradation
DOI:
doi:10.5061/dryad.4bq08
摘要:
ng independent contrasts analysis. We found some evidence that extracted DNA concentration is correlated with body size and inversely correlated with time since collecti
Data from: DNA extraction method affects the detection of a fungal pathogen in formalin-fixed specimens using qPCR
负责人:
关键词:
DNA extraction frogs amphibians amphibian decline natural history collections disease chytridiomycosis
DOI:
doi:10.5061/dryad.060tg
摘要:
to detect Bd in formalin-fixed specimens after three years of ethanol storage. Our objectives were to compare the most commonly used DNA extraction method
Data from: Improving PCR detection of prey in molecular diet studies: importance of group-specific primer set selection and extraction protocol
负责人:
Zarzoso-Lacoste, Diane
关键词:
Invasive rats Diet Analysis PCR-based method Extraction protocol performances A260/A280 absorbance ratio Group-specific primer pairs
DOI:
doi:10.5061/dryad.8c134
摘要:
selected to be use as group-specific primer pairs in PCR protocols. The performances of four DNA extraction protocols (QIAamp DNA stool mini kit, DNeasy

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