SIEKMANN, JUERGEN,WEBER, ALFRED,ROTTENSTEINER, HANSPETER,TURECEK, PETER
申请号：
CA3012117
公开号：
CA3012117A1
申请日:
2012.05.25
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
The present invention features a method of preparing a therapeutic proteinconjugate. The method comprises contacting a therapeutic protein orbiologically-active fragmentthereof with a thiol reductant and a polysialic acid (PSA) or a functionalderivative thereof, underconditions that (a) produce a reduced cysteine sulfhydryl group on thetherapeutic protein, theprotein having no more than one accessible cysteine sulhydryl group, and (b)allow conjugation ofthe PSA to the reduced cysteine sulfhydryl. The therapeutic protein is aprotein of the serpinsuperfamily, or a protein selected from any of: antithrombin III, alpha-1-antichymotrypsin,human serum albumin, alcohol dehydrogenase, biliverdin reductase, buturylcholinesterase,complement C5a, cortisol-binding protein, creatine kinase, ferritin, heparincofactor, interleukin 2,protein C inhibitor, tissue factor； vitronectin； ovalbumin, plasminogen-activator inhibitor,neuroserpin, C1-Inhibitor, nexin, alpha-2-antiplasmin, heparin cofactor II,alphal-antichymotrypsin, alphal-microglobulin； protein C, protein S, tPA, PAI-1,tissue factor (TF) or ADAMTS 13 protease.
