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Toxoid, Compositions and Related Methods
专利权人:
发明人:
Mark Shieh,Mike Soika
申请号:
US14776189
公开号:
US20160030542A1
申请日:
2014.03.14
申请国别(地区):
US
年份:
2016
代理人:
摘要:
Methods of producing purified clostridial toxin comprising tangential flow filtration, hydrophobic interaction chromatography and anion exchange chromatography are disclosed. These methods provide good yields of C. difficile toxin having a purity of about 90% or greater. Highly purified Clostridial toxins, toxoids (e.g., prepared by inactivating the toxin as disclosed herein) and compositions comprising these toxins and/or toxoids are also disclosed. Methods of using the purified toxins and/or toxoids for example, to elicit an immune response against Clostridium (e.g., C. difficile) are also disclosed.
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