The present invention relates to a system for separating, optionally expanding, and concentrating multipotent stromal cells (MSCs). The concentrated MSC are ready for administration to a patient in need of MSCs to repair tissue damage. The system according to the invention is a closed system which does not require centrifugation steps to obtain isolated and concentrated MSCs. Therefore the risk of external contamination is minimized. The present invention also relates to a method of obtaining isolated, optionally expanded, concentrated MSCs using the system according to the invention.