The components and methods provided include polynucleotides and polypeptides with activity of EPSP (5-enolpiruvilshikimato-3-phosphate) Sinteso (EPSPS). Among the concrete results,Sequences have a better property.Like,But not limited to,Enhance catalytic activity in the presence of inhibitors.Glycerol. Nucleic acid engineering is also provided.Botany,plant cellExplainSeeds and cereals, which have EPSPS sequences. Various methods are provided for the use of EPSPS sequences. These methods include the production of glycerol-tolerant plants.plant cell1. The methods for sowing crops and/or seeds and controlling weeds described in this document. Requirement 1: a polnuclear pollution codification of EPSP manipulation plant rate (EPSPS),The EPSPS polypeptide of this plant includes G102A and at least one or more amino acid mutations selected from the A2R group.A2,G3K,A4w,S38A,H54L,H54G,A69H,K71E,K84R,E92G,L98C,N162R,I208L,R216V,K224R,E226Y,K243L,K243E,M293L,K297A,E302P,V333A,A354G,T361S,R368C,E391P,R429A,D402G and A416G,Among them, each mutation position of amino acid is consistent with the position of amino acid determined in SEQ ID 1. EPSPS polypeptide in this substance includes a series of amino acids, which are at least 90% the same as SEQ ID 2.Se proporcionan composiciones y métodos que comprenden polinucleótidos y polipéptidos que tienen actividad de EPSP (5-enolpiruvilshikimato-3-fosfato) sintasa (EPSPS). En realizaciones específicas, la secuencia tiene una propiedad mejorada, tal como, pero no limitada a, capacidad catalítica mejorada en la presencia del inhibidor, glifosato. Adicionalmente se proporcionan construcciones de ácido nucleico, plantas, células vegetales, explantes, semillas y granos que tienen las secuencias de EPSPS. Se proporcionan diversos métodos para emplear las secuencias de EPSPS. Dichos métodos incluyen métodos para producir una planta tolerante a glifosato, célula vegetal, explante o semilla y métodos para controlar malezas en un campo que conti
