Disclosed are mammalian tumor and/or cancer cell conditioned substrate preparations having tumor inhibiting activity. In some embodiments, the mammalian tumor and/or cancer cell conditioned substrate preparations are essentially free of cellular components. These preparations comprise a conditioned heterologous acellular collagenous tissue preparation, and may be prepared using a mammalian extracellular matrix substrate. The conditioned substrates include many different anti-tumor and/or anti-cancer biomolecules, such as that population of anti-tumor biomolecules that are secreted and/or produced by mammalian tumor and/or cancer cells as they grow on a substrate, thus imparting the anti-tumor and/or anti-cancer properties to the conditioned substrates of the invention. The present disclosure also provides methods for preparing the mammalian tumor and/or cancer cell conditioned substrates, as well as methods for using the preparations to inhibit tumor growth, such as in a vaccine or wound dressing. Methods for inhibiting prostate tumors and melanoma with the described conditioned substrates are also described. The conditioned mammalian tumor and/or cancer cell substrate preparations are essentially free of viable and/or carcinogenic or invasive tumor and/or cancer cells. The conditioned substrates and substrate preparations include anti-tumor and anti-cancer properties that may be used in preparations and formulations for the treatment of cancer.Linvention concerne des préparations de substrat conditionné par des cellules tumorales et/ou cancéreuses de mammifère présentant une activité dinhibition des tumeurs. Dans certains modes de réalisation, les préparations de substrat conditionné par des cellules tumorales et/ou cancéreuses de mammifère sont essentiellement dépourvues de composants cellulaires. Ces préparations comprennent une préparation de tissu collagénique acellulaire hétérologue conditionné, et peuvent être préparées en utilisant un substrat de matrice ext
