A method for protein refolding with an ion exchange resin. The method includes (a) choosing an ion exchange resin that having charged groups with the same sign as a net charge of a denatured protein to be refolded (b) removing heterogeneous ions from the ion exchange resin by washing the ion exchange resin sequentially with saline solution and deionized water, to prepare the ion exchange resin (c) mixing the ion exchange resin with a refolding buffer thoroughly, then adding the denatured protein to the refolding buffer and allowing the denatured protein to refold and then (d) collecting the supernatant by centrifugation or settlement, to obtain a solution containing the refolded protein.
