The present invention relates to a queen bee blocking member for a native bee and a queen bee management device and a queen bee management method using the queen bee blocking member for a native bee and, more particularly, to a queen bee blocking member for a native bee and a queen bee management device and a queen bee management method for a native bee using the queen bee blocking member for a native bee regulating mating and spawning seasons based on queen bee entrance control over a certain period of time so that viral infection leading to sacbrood can be fundamentally blocked, native honey productivity can be significantly enhanced based on natural swarming blocking and regulation, queen bee spawning regulation and management by flowering season, and the blocking of an invader bee (harmful insect) such as a bumble bee and a foreign bee, and efficient queen bee multiplication can be ensured, the queen bee blocking member for a native bee being capable of being used for replacement and storage purposes. The queen bee management device for a native bee according to an embodiment of the present invention includes: a support having a supporting plate and a supporting frame stacked on an upper surface of the supporting plate for one side to be open and disposed at a predetermined distance from a bottom surface a hive stacked on the support and having a lower box and an upper box having open lower and upper portions a barrel hive where the hive is integrally connected to the support or a cover member mounted on the hive and at least one first queen bee blocking member provided in the support and the hive and having multiple queen bee blocking slits for queen bee separation or a second queen bee blocking member capable of queen bee separation or mating blocking, regulation, and control with multiple queen bee blocking slits formed in lower end portions of the support and the hive.COPYRIGHT KIPO 2017본 발명은 토종벌의 여왕벌 차단부재와 이를 이용한 여왕벌 관리장치 및 그 방법에 관한 것으로, 특히 일정 기간 여왕벌의 출입 통제