An apparatus for acquiring bioinformation, including a laser irradiator configured to irradiate a laser beam onto a region of interest including blood vessels a sensor configured to detect a first change in a laser speckle pattern generated by the laser beam reflected from the region of interest and a processor configured to acquire a biosignal indicating a second change in blood flow within the blood vessels based on the detected first change in the laser speckle pattern, and to acquire the bioinformation by using the biosignal, wherein the laser beam emitted from the laser irradiator is incident at an angle to the region of interest.